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Isolation and characterization of squalene synthase cDNA fromcentella asiatica (L) urban

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We have cloned and characterized a gene for squalene synthase (SQS) fromCentella asiatica (L) Urban, a species that produces a large quantity of triterpene saponins such as asiaticoside and madecassoside. Its full-length cDNA clone was isolated by RACE PCR. The sequence ofpSQS contains an open reading frame of 1248 nucleotides, which code for 416 amino acids with a molecular mass of 47.3 kDa. Southern analysis revealed that one copy might exist in the C.asiatica genome. We also determined that 0.1 mM methyl jasmonate was sufficient to up-regulate those levels ofCaSQS mRNA.

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Correspondence to Baik Hwang.

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Kim, O.T., Seong, N.S., Kim, M.Y. et al. Isolation and characterization of squalene synthase cDNA fromcentella asiatica (L) urban. J. Plant Biol. 48, 263–269 (2005).

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