Genetic transformation ofAjuga multiflora bunge withAgrobacterium rhizogenes and 20-hydroxyecdysone production in hairy roots

Abstract

An efficient transformation system forAjuga multiflora Bunge was established by usingAgrobacterium rhizogenes strain A4. After inoculation with the bacteria, we obtained a number of hairy-root clones from micro-calli of the explant petioles. One fast-growing line showed the highest production of 20-hydroxyecdysone (20-HE). PCR amplification of rooting locus (rol) genes revealed that the left hand-transferred DNA of the root-inducing plasmid was inserted into the genome of our transformedAjuga hairy roots. This integration was further confirmed by DNA-DNA hybridization. The 20-HE content in hairy roots was 10 times higher than that measured in the wild type.

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Correspondence to Ok Tae Kim or M. Manickavasagm or Young Jun Kim or Mei Ran Jin or Kwang Soo Kim or Nak Sul Seong or Baik Hwang.

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Kim, O.T., Manickavasagm, M., Kim, Y.J. et al. Genetic transformation ofAjuga multiflora bunge withAgrobacterium rhizogenes and 20-hydroxyecdysone production in hairy roots. J. Plant Biol. 48, 258–262 (2005). https://doi.org/10.1007/BF03030416

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Keywords

  • Ajuga multiflora
  • hairy roots
  • 20-hydroxyecdysone
  • Ri-plasmid
  • rol genes