Abstract
We investigated the optimal levels of growth regulators, culture media, and pH on callus growth and organogenesis of in-vitro cultured ‘Kyoho’ grapes. Calli were induced by culturing leaf blades on an MS basal medium supplemented with 1 mg/IL BA and 0.01 mg/L 2,4-D. In addition, calli originating from the exocarp and mesocarp of grape fruits devel-oped on MS media supplemented with 0.1 mg/L IAA, NAA, or 2,4-D, or with 0.2 mg/L BA. In testing the potential for plant regeneration from shoot tips on various media, we found that the Nitsch medium, with I mg/L BA, was optimal for caulogenesis. The type of shoot development depended on the pH of the medium, with vigorous multiple-shoot devel-opment occurring at pH 6.0, and single shoots forming at pH 5.0. Finally, we were able to obtain rooted seedlings from the regenerated shoots that had been cultured on 1/4-strength Nitsch medium supplemented with 0.03 mg/L NAA.
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Literature Cited
Chee R, Pool RM (1983) In vitro propagation ofVitis: Application of previously defined culture condition to a selection of genotypes. Vitis22: 363–374
Chee R, Pool RM (1985) In vitro propagation ofVitis: The effects of organic substances on shoot multiplication. Vitis24: 106–118
Chee R, Pool RM (1987) Improved inorganic media constituents for in vitro shoot multiplication ofVitis. Scientia Horticulturae32: 85–95
Chi B, Francois C (1991) Effects of high ammonium concentrations on growth and anthocyanin formation in grape(Vitis vinifera L.) cell suspension cultured in a production medium. Plant Ceil Tiss Org Cult27: 169–174
Choi IM, Hyung NI, Kim SB (1992) Effects of medium compositions on shoot multiplication and rooting in shoot tip cultures of ‘Kyoho’ and ‘Campbell Eariy’ grapes. Kor J P]ant Tiss Cult19: 59–66
Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi EY (1975) Establishment of an efficient medium for anther culture of rice, through comparative experiments on the nitrogen sources. Scientia Sinic18: 659–668
Dodds JH, Roberts LW (1985) Initiation and maintenance of callus,In JH Dodds, LW Roberts, eds, Experiments in PLant Tissue Culture, Ed 2, Cambridge and New York, pp57
Emershad RL, Ramming DW (1994) Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (Vitisvinifera L.). Plant Cell Rep14: 6–12
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Ceil Res50: 151–158
Gray DJ (1992) Somatic embryogenesis and plant regeneration from immature zygotic embryos of Muscadine grape(Vitis rotundifolia) cuItivars. Amer J Bot79: 542–546
Gray DJ, Benton CM (1991) In vitro micropropagation and plant establishment of muscadine grape cuItivars(Vitis rotundifolia). Plant Cell Tiss Org Cult27: 7–14
Gresshoff PM, Doy CH (1974) Derivation of a haploid cell line fromVitis vinifera and the importance of the stage of meiotic development of anthers for haploid culture of this and other genera. Z Pflanzenphysiol73: 132–141
Hyung NI, Kang SK, Lee CH (1992) Protoplast isolation and culture of Grapes cv ‘Cabernet Sauvignon’ (Vitis vinifera L) and ‘Niagara’(Vitis Iabmsca L.). Kor J Pfant Tiss Cult19: 227–231
Isabetle GT, Mauro MC, Sossountzov L, Miginiac E, Deioire A (1993) Arrest of somatic embryo development in grapevine: histologicaI characterization and the effect of ABA, BAP and zeatin in stimulating plantiet development. Plant Cell Tiss Org Cult33: 91–103
Kao KN, Michayluk MR (1975) Nutritional requirements for growth ofVicia hajastana cells and protop]asts at a very Iow population density in liquid media. Planta126: 105–110
Kwon Y, Lee CH, Hyung NI (2000) Effect of medium composition and culture condition on plant regeneration via organogenesis of ‘Kyoho’ grape. J Kor Soc Hort Sci41: 276–280
Lichter R (1981) Anther culture ofBrassica napus in a liquid culture medium. Z Pflanzenphysio1103: 22–237
Lloyd G, McCown B (1980) Commercially feasible micro-propagation of mountain laurel,Kalmia latifolia, by use of shoot-tip culture. Int Plant Prop Soc Proc30: 421–427
Mozsar J, Sule S (1994) A rapid method for somatic embryogenesis and plant regeneration from cultured anthers ofVitis riparia. Vitis33: 245–246
Murashige -r, Skoog F (1962) A received medium for rapid growth and bio-assays with tobacco tissue cultures. Physio J Plant15: 473–497
Nakano, Sakakibara MT, Watanabe Y (1997) Establishment of embwogenic cultures in several cultivars ofVitis vinifera andV. ×labmscana. Vitis36: 141–145
Nitsch JP (1969) Experimental androgenesis inNicotiana. Phytomorph19: 389–404
Perl A, Saad S, Sahar N, Holland D (1995) Establishment of long-term embwogenic cultures of seedlessVitis vinifera cultivars — a synergistic effect of auxins and the role of abscisic acid. Plant Sci104: 193–200
Perrin M, Martin D, Joly D, Demangeat G, This P, Masson JE (2001) Medium-dependent response of grapevine somatic embwogenic cells. Plant Sci161: 107–116
Reustle G, Harst M, Alleweldt G (1994) Regeneration of grapevine(Vitis sp.) protoplasts. Vitis33: 173–174
Sasahara H, Tada K, Iri M, Takezawa T, Tazaki M (1981) Regeneration of plantlet by meristem tip culture for virus-free grapevine. J Jap Soc Hort Sci80: 169–175
Stamp JA, Colby SM, Meredith CP (1990) Direct shoot organogenesis and plant regeneration from leaves of grape(Vitis spp.) Plant Cell Tiss Org Cult22: 127–133
Torregrosa L, Montserrat TV, Bouquet A (1995) Somatic embryogenesis from leaves ofVitis × Muscadinia hybrids. Vitis34: 239–240
Tortes KC (1989) Overview of facilities and techniques,In KC Torres, ed, Tissue Culture Techniques for Horticultural Crops, van Nostrand Reinhold, New York, pp 22–23
Zatyko JM, Molnar I (1990) Adventitious root formation of chokeberry(Arola melanocarpa Elliot) influenced by the pH of medium. Fruit Sci Rep17: 21–27
Zhu YM, Hoshino Y, Nakano M, Takahashi E, Nil M (1997) Highly efficient system of plant regeneration from protoplast of grapevine(Vitis vinifera L.) through somatic embwogenesis by using embwogenic callus culture and activated charcoal. Plant Sci123: 151–157
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Park, HJ., Lee, HR., Pyee, J. et al. Regeneration of grape (Vitis labruscana cv. Kyoho) by shoot-tip culture. J. Plant Biol. 44, 185–192 (2001). https://doi.org/10.1007/BF03030350
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DOI: https://doi.org/10.1007/BF03030350