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Phylogeny of the genusGoodyera (Orchidaceae; Cranichideae) in Korea based on nuclear ribosomal DNA ITS region sequences

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Abstract

We sequenced the nuclear ribosomal internal transcribed spacer (ITS) regions to determined the phylogenetic relationships of the severalGoodyera species in Korea and to measure the extent of their differentiation region. ITS 1 was 238 to 239 bp long while ITS 2 was 258 to 259 bp. The 5.8S coding region was 156 bp long. Sequence divergences among species, calculated by Kimura’s two- parameter method, ranged from 0.0 to 5.4%. The most parsimonious tree, with a consistency index of 0.935 and a retention index of 0.937, was produced with 337 steps. Our ITS sequence results demonstrate the monophyly of KoreanGoodyera and support previous morphological, geographical, and RAPD data analyses.

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Authors and Affiliations

  1. Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 305-333, Daejon, Korea

    Kee-Sun Shin

  2. Division of Genome Resources and Reservation, National Genome Research Institute, National Institute of Health, 122-701, Seoul, Korea

    Yong Kook Shin

  3. Division of Life Science, Daejeon University, 300-716, Daejon, Korea

    Joo-Hwan Kim

  4. Dongbu Research Council, Korea Native Plants Institutes, Daeduck Science Town, 305-333, Daejon, Korea

    Kyoung-Hwan Tae

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  1. Kee-Sun Shin
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  2. Yong Kook Shin
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  3. Joo-Hwan Kim
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  4. Kyoung-Hwan Tae
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Correspondence to Kyoung-Hwan Tae.

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Shin, KS., Shin, Y.K., Kim, JH. et al. Phylogeny of the genusGoodyera (Orchidaceae; Cranichideae) in Korea based on nuclear ribosomal DNA ITS region sequences. J. Plant Biol. 45, 182–187 (2002). https://doi.org/10.1007/BF03030312

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  • DOI: https://doi.org/10.1007/BF03030312

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