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Activation of ascorbate-glutathione cycle inArabidopsis leaves in response to aminotriazole

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Abstract

Aminotriazole(AT)-induced changes in growth, hydrogen peroxide content and activities of H2O2-scavenging antioxidant enzymes were investigated in the growing leaves ofArabidopsis plants (Arabidopsis thaliana cv Columbia). Catalase activity of rosette leaves was reduced by 65% with an application of 0.1 mM AT (a herbicide known as a catalase inhibitor), whereas the leaf growth and H2O2 content were almost unaffected. However, an approximate 1.6 to 2-fold increase in cytosolic ascorbate peroxidase (APX) activity concomitant with a substantial activation of glutathione reductase (GR) (approx. 22% increase) was observed during leaf growth in the presence of 0.1 mM AT. The activity of cytosolic APX in leaves was also increased by 1.8-fold with an application of exogenous 2 mM paraquat (an inducer of H2O2 production in plant cells) in the absence of AT. These results collectively suggest that (a) cytosolic APX and GR operate to activate an ascorbate-glutathione cycle for the removal of H2O2 under severe catalase deactivation, and (b) the expression of APX seems to be regulated by a change of the endogenous H2O2 level in leaf cells.

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Authors and Affiliations

  1. Division of Biology, Kangwon National University, 200-701, Chuncheon, Korea

    Kyong-Suk Kang, Chang-Jin Lim, Tae-Jin Han, Joon-Chul Kim & Chang-Duck Jin

  2. Department of Biology, Hallym university, 200-702, Chuncheon, Korea

    Tae-Jin Han

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  1. Kyong-Suk Kang
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  2. Chang-Jin Lim
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Kang, KS., Lim, CJ., Han, TJ. et al. Activation of ascorbate-glutathione cycle inArabidopsis leaves in response to aminotriazole. J. Plant Biol. 41, 155–161 (1998). https://doi.org/10.1007/BF03030248

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  • DOI: https://doi.org/10.1007/BF03030248

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