Abstract
Human interleukin-2 (IL-2) gene was inserted next of the 35S-transcript promoter of cauliflower mosaic virus in a binary vector and introduced into tobacco genome byAgrobacterium- mediated transformation. Genomic DNA blot analysis of thein vitro regenerated transgenic tobacco confirmed that the introduced human IL-2 gene was successfully integrated into tobacco genome. RNA blot analyses were carried out for the IL-2 transcript to examine expression of the introduced IL-2 gene. Specific signals only from the transgenic tobacco plants indicated that the introduced human IL-2 gene was properly transcribed. The transgenic tobacco plants were self-pollinated and the seeds were harvested. Differences in the level of the transcript were more apparent during the germination of the seeds and between the T2 generation tobacco plants.
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Park, S.M., Hong, C.B. Transcription of human lnterleukin-2 gene inNicotiana tabacum driven by CaMV35S promoter. J. Plant Biol. 40, 110–114 (1997). https://doi.org/10.1007/BF03030242
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DOI: https://doi.org/10.1007/BF03030242