Abstract
In order to clarify the influence of low humidity culture on the structure and function of somatic embryo the cotyledon expiants ofDaucus carrota L. cv. Hongshim were cultured in the petridish whose lids had holes sealed with millipore filters. In the low humidity culture, the production of somatic embryos was enhanced and their maturation promoted but the cotyledon structure of somatic embryos were nearly similar to control. In addition, the low humidity culture improved the germination of somatic embryos. Especially, the germination frequency of jar-shaped embryos was much improved (68%) in comparison with that of jarshaped embryos formed in constant humidity culture (23%). But low humidity culture at its extreme became an obstacle to normal plant regeneration in that precocious embryos were generated and the primary embryos turned into callus and formed secondary embryos. Therefore it is suggested that moderately low humidity culture (80–90% R.H.) is important to the higher production and better-quality of somatic embryos.
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Literature Cited
Ammirato, P.V. and F.C. Steward. 1971. Some effects of the environment on the development of embryos from cultured free cells.Bot. Gaz.132: 149–158.
Ammirato, P.V. 1983. The regulation of somatic embryo development in plant cell cultures: suspension culture techniques and hormone reguirements.Biotechnology1: 68–74.
Attree, S.M., D. Moore, V.K. Sawhney and L.C. Fowke. 1991. Enhanced maturation and desiccation tolerance of while spruce(Picea glauca [Moench.] Voss) somatic embryos: Effects of a non-plasmolysing water stress and abscisic acid.Ann. Bot.68: 519–525.
Buchheim, J.A., S.M. Colburn and J.P. Ranch. 1989 Maturation of soybean somatic embryos and the transition to plantlet growth.Plant Physiol.89: 768–775.
Burns, J.A. and H.Y. Wetzstein. 1995. Development and germination of pecan somatic embryos derived from Liquid Culture.In Vitro Cell. Dev. Biol.31: 72- 78.
Cho, D.Y. and W.Y. Soh. 1995. Morphological observation of somatic embryogenesis in leaf culture ofBupleurum falcatum L.Korean J. Plant Tiss. Cult.22: 291–297.
Choi, P.S., W.Y. Soh, D.Y. Cho and J.R. Liu. 1994a Somatic embryogenesis in immature zygotic embryo cultures of Korean soybean(Glycine max L.) culticars and effect of 2.4-dichlorophenoxyaeetic acid on somatic embryo morphology.Korean J. Plant Tiss. Cult.21: 7–13.
Choi, P.S., W.Y. Soh and J.R. Liu. 1994b. Effects of carbohydrate sucrose and osmotium on the structural abnormality of somatic embryo ofCondopsis lanceolata. Abstract. Spring Meet. Kor. Soc. Plant Tiss. Cult., p. 47.
Kamada, H., K. Kobayashi, T. Kiyosue and H. Harada. 1989. Stress induced somatic embryogenesis in carrot and its application to synthetic seed production.In Vitro Cell. Dev. Biol.25:1163–1166.
Kazuko, Y.S. and S. Kazuko. 1994. A novel cis-acting element in an arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress.Plant Cell6: 251–264.
Kermode, A.R. and D.J. Bewley. 1985. The role of maturation drying in the transition from seed development to germination.J. Exp. Bot.36: 1916–1927.
Kermode, A.R. and D.J. Bewley. 1989. Developing seeds ofRiccinus communis L. when detached and maintained in an atmosphere of high relative hymidity, switch to a germinative mode without the requirement for complete desiccation.Plant Physiol.90: 702–707.
Kermode, A.R. 1990. Regulatory mechanisms involved in the transition from seed development to germination.Plant Sci.9: 155–195.
Lee, K. S. 1993. Structural diversity of cotyledons of somatic embryos from cultured cell ofAralia cordata Thunb.Dissertation, Graduate School, Chonbuk National University, Chonju, Korea, 86 pp.
Lee, K.S. and W.Y. Soh. 1993a. Somatic embryogenesis and structural aberrancy of embryos in tissue cultures ofAralia cordata Thunb.Korean J. Plant Tiss. Cult.20: 77–83.
Lee, K.S. and W.Y. Soh. 1993b. Effects of cytokinins on the number of cotyledons of somatic embryos from cultured cells ofAralia cordata Thunb.Korean J. Plant Tiss. Cult.20: 171–175.
Lee, K.S. and W.Y. Soh. 1994. Effects of abscisic acid on the number of somatic embryo cotyledons in tissue culatures ofAralia cordata Thunb.Korean J. Plant Tiss. Cult.5: 287–291.
Mathews, H., C. Schopke, R. Carcamo, P. Chavarriaga, C. Fauguet and R.N Beachy. 1993. Improvement of somatic embryogenesis and plant recovery in cassava.Plant Cell Rep.12: 328–333.
Philippe, V., Y. Horth and F. Pascal. 1989 Enhancement of production and regeneration of embryogenic type II callus inZea mays L. by AgNO3.Plant Cell Tiss. Org. Cult.18: 143–151.
Roberts D.R., W.R. Lazaroff and F.B. Webster. 1991 Interaction between maturation and high relative humidity treatments and their effects on germination of sitka spruce somatic embryos.J. Plant Physiol.138: 1–6.
Roustan, J.P., A. Latche and J. Fallot. 1989. Stimulation ofDaucus carrota somatic embryogenesis by inhibitors of ethylene synthesis: cobalt and nickel.Plant Cell Rep.8: 182–185.
Saito, T., S. Nishizawa and S. Nishimura. 1991. Improved culture conditions for somatic embryogenesis fromAsparagus officinalis L. using an aseptic ventilative filter.Plant Cell Rep.10: 230–234.
Soh, W.Y. 1993. Developmental and structural diversity of regenerated plants in cell and tissue cultures. In; Molecular Approach to Plant Cell Differentiation.Proc. 7th Symp. Plant Biotech. Bot. Soc. Korea, pp. 1–35.
Soh, W.Y., D.Y. Cho and E.K. Lee. 1996. Multicotyle- donary structure of somatic embryo formed from cell cultures ofDaucus carota L.J. Plant Biol.39: 71–77.
Veramendi, J. and L. Navarro. 1996. Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm.Plant Cell Tiss. Org. Cult.45: 159–164.
Wetzstein, H.Y. and C.M. Baker. 1993. The relationship between somatic embryo morphology and conversion in peanut(Arachis hypogaea L.).Plant Sci.92: 81–89.
Yehoshua, S., D. Rhodes and J. Janick. 1992. Changes in amino acid composition associated with tolerance to partial desiccation of celery somatic embryos.J. Amer. Soc. Hort. Sci.117: 337–341.
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Lee, E.K., Cho, D.Y. & Soh, W.Y. Effect of humidity on somatic embryogenesis in cotyledon explant culture of carrot. J. Plant Biol. 40, 89–94 (1997). https://doi.org/10.1007/BF03030239
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DOI: https://doi.org/10.1007/BF03030239