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High-performance liquid chromatographic assay of serotonin in human plasma

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Ricerca in clinica e in laboratorio

Summary

A sensitive and reproducible reversed-phase high-performance liquid chromatography with electrochemical detection for determination of serotonin in human plasma is described. The method has an average coefficient of variation of 3.5%; the recovery of serotonin and 5-hydroxy-N-methyltryptamine (internal standard) accounts for 95±2%. Average serotonin concentrations in platelet-free plasma and in platelet-rich plasma from 21 normal subjects (22-63-year-old) ranged from 0.6 to 4.9 ng/ml (mean 2.8±1.3 SD) and from 38.3 to 106.5 ng/108 platelets (mean 66.8±18.9 SD), respectively. The release of endogenous serotonin from human aggregating platelets challenged by collagen or adenosine diphosphate has been quantitated.

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Authors and Affiliations

  1. Istituto di Scienze Biomediche, Clinica Medica V Ospedale S. Paolo, Via di Rudinì 8, 20142, Milano, Italy

    Bianca Marashini, Maria Luisa Biondi & Angelo Agostoni

  2. Dipartimento di Scienze e Tecnologie Biomediche, Sezione di Chimica Organica, Università degli Studi di Milano, Italy

    Piergiorgio Pietta

Authors
  1. Bianca Marashini
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  2. Maria Luisa Biondi
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  3. Piergiorgio Pietta
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  4. Angelo Agostoni
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Additional information

This work was partially supported by a grant (contract no. 83.02081.04) from theConsiglio Nazionale delle Ricerche (CNR), Roma, Italy.

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Marashini, B., Biondi, M.L., Pietta, P. et al. High-performance liquid chromatographic assay of serotonin in human plasma. La Ricerca Clin. Lab. 15, 63–69 (1985). https://doi.org/10.1007/BF03029163

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  • DOI: https://doi.org/10.1007/BF03029163

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