Effect of ischemic post-conditioning on spinal cord ischemic-reperfusion injury in rabbits

Effet du post-conditionnement ischémique sur les lésions d’ischémie-reperfusion de la moelle épinière chez le lapin

Abstract

Objectives

To investigate the potential protective effect of ischemic post-conditioning (Post-con) on ischemia-reperfusion injury of the rabbit spinal cord, and to determine if there is an additive neuroprotective effect when ischemic preconditioning (IPC) and Post-con are combined.

Methods

Forty New Zealand white rabbits were randomly divided into four groups: group Control (C;n = 10), aortic occlusion (AOC; for 30 min; group IPC (n = 10) three cycles of three-minute AOC plus three-minute reperfusionbefore the 30-min AOC; group Post-con (n = 10), three cycles of three-minute reperfusion plus three-minute AOC immediately upon reperfusionafter 30-min AOC; group IPC+Post-con (n = 10), where animals were subjected to both IPC and Post-con. At six hours, 24 hr and 48 hr following reperfusion, neurological function was assessed according to Tarlov scores, and at 48 hr, the spinal cords were procured for the histopathologic evaluation, by comparing the number of intact α-motor neurons in the anterior horn.

Results

The median count (and quartiles) of intact α-motor neurons was greatest in group Post-con 73 (69–76) and group IPC+Post-con 29 (22–42) compared to the numbers of viable α-motor neurons in groups C 6 (4–9) and IPC 15 (11–18) (P <01). The numbers of animals who developed paraplegia according to Tarlov criteria were 7/10 in groups Post-con and IPC+Post-con, compared to 9/10 animals in each of groups C and IPC.

Conclusions

This laboratory investigation provides histological evidence that Post-con may protect the spinal cord from moderate to severe ischemia reperfusion injury. Ischemic preconditioning conferred no additional benefits in this rabbit model. The results have potential clinical implications for patients undergoing thoracoabdominal aortic reconstructive surgery.

Résumé

Objectif

Investiguer l’et protecteur potentiel du post-con-ditionnement ischémique (Post-con) sur les lésions d’ischémie-reperfusion de la moelle épinière chez le lapin, et déterminer s’il y a un effet neuro-protecteur lors de la combinaison du pré-conditionnement ischémique (PCI) et du Post-con.

Méthode

Quarante lapins blancs de Nouvelle-Zélande ont été répartis en quatre groupes de façon aléatoire: groupe témoin (T; n = 10), occlusion aortique (OCA) de 30 min; groupe PCI (n = 10), trois cycles de reperfusion de trois minutes plus OCA de trois minutes immédiatement à la reperfusion après une OCA de 30 min; groupe PCI+Post-con (n = 10), dans lequel les animaux ont été soumis à un PCI et un Post-con. Six heures, 24 h et 48 h après reperfusion, la fonction neurologique a été évaluée selon le barème de Tarlov, et à 48 h, les moelles épinières étaient soumises à une évaluation histo-pathologique, en comparant le nombre de neurones moteurs-α intacts au niveau de la corne antérieure.

Résultats

Le nombre médian (et les quartiles) de neurones moteurs-α intacts fut plus élevé dans le groupe Post-con 73 (69–76) et le groupe PCI+Post-con 29 (22–42), que le nombre de neurones moteurs-α viables dans les groupes T 6 (4–9) et PCI 15 (11–18) (P < 0,001). Le nombre d’animaux ayant développé une paraplégie selon les critères de Tarlov était de 7/10 dans les groupes Post-con et PCI+Post-con, comparé à 9/10 animaux dans chacun des groupes T et PCI.

Conclusions

Cette étude en laboratoire offre des preuves histologiques que le Post-con peut protéger la moelle épinière de lésions d’ischémie-reperfusion modérées à sévères. Le pré-conditionnement ischémique n’a pas apporté de bénéfices supplémentaires dans ce modèle chez le lapin. Ces résultats ont des implications cliniques potentielles pour des patients subissant des chirurgies de reconstruction de l’aorte thoraco-abdominale.

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Correspondence to Han Huang or Lan Zhang or Yan Wang or Junyan Yao or Hao Weng or Hai Wu or Zhiyu Chen or Jin Liu.

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Huang, H., Zhang, L., Wang, Y. et al. Effect of ischemic post-conditioning on spinal cord ischemic-reperfusion injury in rabbits. Can J Anesth 54, 42 (2007). https://doi.org/10.1007/BF03021898

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Keywords

  • Spinal Cord
  • Spinal Cord Injury
  • Paraplegia
  • Ischemic Precondition
  • Spinal Cord Ischemia