Endocrine

, Volume 3, Issue 4, pp 245–249 | Cite as

Is there a role for estrogen in follicular maturation in the primate?

  • N. Selvaraj
  • A. S. Bhatnagar
  • N. R. Moudgal
Papers

Abstract

The present study focusses attention on the effects of blocking estrogen synthesis, during follicular phase, on follicular maturation in the adult female bonnet monkey (Macaca radiata). Administration of cycling females (n=4) with an aromatase inhibitor CGS 16949A (Al) by Alzet mini-pump (2,5 mg/day) from day 3 of cycle resulted in significant reduction in basal (by 53%) and surge levels of estrogen (by 70%) but this had no effect on follicular maturation, ovulation and luteal function as assessed by serum hormone profiles as well as laparotomy. This lack of need for estrogen for completion of follicular maturation process was confirmed by administering cycling monkeys hFSH (25 IU/day) from day 3 till day 8 of the cycle along with (5 mg Al/day) or without Al (n=3/group). Administration of AI resulted in suppression of FSH induced increase in serum estrogen (by 100%) and elevation in circulating androstenedione. Aromatase inhibitor treatment had no effect on either the number of follicles developed or their size relative to control. Testing the ability of both granulosa and thecal cells, removed on day 9 of treatment cycle, to respond to gonadotropinsin vitro showed no change indicating that cellular development and maturation of follicular cells had occurred normally. It is concluded that follicular maturation in the primate can occur even when increase in estrogen synthesis is blocked.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adashi, E.Y. & Hsueh, A.J.W. (1982).J. Biol. Chem.,257, 6077–6083.PubMedGoogle Scholar
  2. Bhatnagar, A.S., Schieweck, K., Hausier, A., Browne, L.J. & Steele, R.E. (1989).Proceedings of the Rovai Society of Edinburgh.,95B, 293–303.Google Scholar
  3. Billiar, R.B., Loukides, J.A. & Miller, M.M. (1992).J. Clin. Endocrinol. Metab.,75, 1159–1165.PubMedCrossRefGoogle Scholar
  4. Channing, C.P. (1980).Endocrinology,107, 342–352.PubMedGoogle Scholar
  5. Goodman, A.L. & Hodgen, G.D. (1983).Recent Prog. Horm. Res.,39, 1–73.PubMedGoogle Scholar
  6. Hausier, A., Schenkel, L., Krahenbuhl, C., Monnet, G. & Bhatnagar, A.S. (1989).J. Steroid Biochem.,33, 125–131.CrossRefGoogle Scholar
  7. Hild-Petito, S., Stouffer, R.L. & Brenner, R.M. (1988).Endocrinology,123, 2896–2905.PubMedGoogle Scholar
  8. Hillier, S.G. (1990).J. Endocrinol.,127, 1–4.PubMedCrossRefGoogle Scholar
  9. Hiller, S.G., Harlow, C.R., Shaw, H.J., Wickings, E.J., Dixon, A.F. & Hodges, J.K. (1987).The primate ovary. Stouffer, RL. (ed). Plenum Press: New York, pp. 61–72.Google Scholar
  10. Holt, J.A., Schumacher, G.F.B., Jacobson, H. & Swartz, D.P. (1979).Fertil. Steril.,32, 170–176.PubMedGoogle Scholar
  11. Koering, M.J. (1987).The primate ovary. Stouffer, RL (ed). Plenum Press: New York, pp. 3–15.Google Scholar
  12. Krietmann, O., Nixon, W.E. & Hodgen, G.D. (1981).Fertil. Steril.,35, 671–675.Google Scholar
  13. Moudgal, N.R., Ravindranath, N., Murthy, G.S., Dighe, R.R., Aravindan, G.R. & Martin, F. (1992).J. Reprod. Fertil.,96, 91–102.PubMedGoogle Scholar
  14. Nasir-ud-din, Jeanloz, R.W., Roussel, P. & Arthur Mc., J.W. (1983).Biol. Reprod.,28, 1189–1199.PubMedCrossRefGoogle Scholar
  15. Pariente, C., Rabinovici, J., Lunenfeld, B., Rudak, E., Dor, J., Mashiach, S., Lavran, D., Blankstein, J. & Geier, A. (1990).J. Clin. Endocrinol. Metab.,71, 984–987.PubMedGoogle Scholar
  16. Prahalada, S., Mukku, V.R., Rao, A.J. & Moudgal, N.R. (1975).Contraception,12, 137–147.PubMedCrossRefGoogle Scholar
  17. Rabinovici, J., Blankstein, J., Goldman, B., Rudak, E., Dor, Y., Pariente, C., Geier, A., Lunenfeld, B. & Mashiach, S. (1989).J. Clin. Endocrinol. Metab.,68, 693–697.PubMedGoogle Scholar
  18. Ravindranath, N., Sheela Rani, C.S., Martin, F. & Moudgal, N.R. (1989).J. Reprod. Fertil.,87, 231–241.PubMedGoogle Scholar
  19. Richards, J.S. (1975).Endocrinology,97, 1174–1184.PubMedGoogle Scholar
  20. Richards, J.S. (1980).Phvsiol Rev.,60, 51–89.Google Scholar
  21. Schenken, R.S., Williams, R.F. & Hodgen, G.D. (1984).Fertil. Steril.,41, 629–634.PubMedGoogle Scholar
  22. Schoot, D.C., Coelingh Bennink, H.J.T., Mannaerts, B.M.J.L., Lamberts, S.W.J., Bouchard, P. & Fauser, B.C.J.M. (1992).J. Clin. Endocrinol. Metab.,74, 1471–1473.PubMedCrossRefGoogle Scholar
  23. Selvaraj, N. (1993). Ph.D. Thesis, Indian Institute of Science, Bangalore, 1–80.Google Scholar
  24. Selvaraj, N. & Moudsal, N.R. (1993).J. Reprod. Fertil.,98, 611–616.PubMedCrossRefGoogle Scholar
  25. Selvaraj, N., Shetty, G., Vijayalakshmi, K., Bhatnagar, A.S. & Moudgal, N.R. (1994).J. Endocrinol., (In press).Google Scholar
  26. Shaw, H.J., Hillier, S.G. & Hodges, J.K. (1989).Endocrinology,124, 1669–1677.PubMedGoogle Scholar
  27. Sheela Rani, C.S. & Moudgal, N.R. (1977).Proc. Ind. Acad. Sci.,87B, 41–51.Google Scholar
  28. Sheela Rani, C.S., Salhanick, A.R. & Armstrong, D.T. (1981).Endocrinology,108, 1379–1385.Google Scholar
  29. Stouffer, R.L., Nixon, W.E., Gulyas, B.J., Johnson, D.K. & Hodges, G.D. (1976).Steroids,27, 543–551.PubMedCrossRefGoogle Scholar
  30. Veldhuis, J.D., Klase, P.A., Strauss J.F. III, & Hammond, J.M. (1982).Endocrinology,111, 144–151.PubMedCrossRefGoogle Scholar
  31. Vidya Shankar, N. & Moudgal, N.R. (1984).J. Bioscience.,6, 263–276.CrossRefGoogle Scholar
  32. Wu, T., Wang, L. & Wan, Y.Y. (1993).Fertil. Steril.,59, 54–59.PubMedGoogle Scholar
  33. Zeleznik, A.J., Keyes, P.L., Menon, K.M.J., Midgley, A.R. Jr. & Reichert, L.E. Jr. (1977).Am. J. Physiol.,233, E229-E234.PubMedGoogle Scholar
  34. Zeleznik, A.J. & Kubik, C.J. (1986).Endocrinology,119, 2025–2032.PubMedGoogle Scholar
  35. Zelinski-Wooten, M.B., Hess, D.L., Wolf, D.P., Stouffer, R.L. (1991). 24th Annual meeting of Society for the study of Reproduction, USA, Abstract 368.Google Scholar
  36. Zelenski-Wooten, M.B., Hess, D.L., Wolf, D.P. & Stouffer, L. (1994).Fertil. Steril.,61, 1147–1155.Google Scholar

Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • N. Selvaraj
    • 1
  • A. S. Bhatnagar
    • 1
    • 2
  • N. R. Moudgal
    • 1
  1. 1.Department of Biochemistry and Primate Research Laboratory, Center for Reproductive Biology and Molecular EndocrinologyIndian Institute of ScienceBangaloreIndia
  2. 2.Ciba-Geigy Ltd.BaselSwitzerland

Personalised recommendations