Abstract
Increasing industrial development in the Masan Bay area of Korea over the past decades increased the risk for the survival of marine organisms in the bay area by the deterioration of the water quality. Since living organisms have the ability to adapt contamination-associated stimuli by the alteration of gene expression, changes in proteins can be used as an important criterion for assessing the levels of environmental conditions. In this study, therefore, alterations of the expression of proteins in the muscle ofLimanda yokohamae from Dukdong and Dotsum in the bay area were surveyed and characterized as compared with Haegumgang, which served as a control site. The results demonstrated that the twenty spots detected from Dukdong and Dotsum were similar to each other. Fifteen proteins were found to be predicted or undefined proteins, while five proteins were identified as heavy polypeptide 11 of myosin, apolipoprotein A-I, fibroblast growth factor 17b precursor, G protein-coupled receptor kinase 1 b and bonnie and clyde. These data suggest that local fish in the bay area have dysfunction in muscle physiology including contraction, lipid metabolism, proliferation and differentiation and nervous system.
Similar content being viewed by others
References
Fenton, S.E. 2006. Endocrine-disrupting compounds and mammary gland development: early exposure and later life consequences.Endocrinology,147(6), 18–24.
Goksoyr, A. 2006. Endocrine disruptors in the marine environment: Mechanisms of toxicity and their influence on reproductive processes in fish.J. Toxicol. Environ. Health A,69, 175–84.
Hong, S.H., U.H. Yim, W.J. Shim, J.R. Oh, and I.S. Lee. 2003. Horizontal and vertical distribution of PCBs and chlorinated pesticides in sediments from Masan Bay, Korea.Mar. Pollut. Bull., 46, 244–253.
Kikuchi, Y., L.A. Trinh, J.F. Reiter, J. Alexander, D.Yelon, and D.Y. Stainier. 2000. The zebra fish bonnie and clyde gene encodes a Mix family homeodomain protein that regulates the generation of endodermal precursors.Genes. Dev.,14, 1279–1289.
Ma, J., X.L Liao, B. Lou, and M.P. Wu. 2004. Role of apolipoprotein A-I in protecting against endotoxin toxicity.Acta. Biochim. Biophys. Sin.,36, 419–424.
MOMAF. 2006. Development of integrated environmental management system for the coastal area of Korea. (1) Ministry of Maritime Affairs and Fisheries, Seoul, Korea. p. 139–409.
Moore, M.N., J.I. Allen, and P.J. Somerfield. 2006. Autophagy: Role in surviving environmental stress.Mar. Environ. Res.,62, 420–5.
Moore, M.N., J.I. Allen, A. McVeigh, and J. Shaw. 2006. Lysosomal and autophagic reactions as predictive indicators of environm e nt a l impact in aquatic animals.Autophagy,2(3), 217–20.
Neuhoff, V., N. Arold, D. Taube, and W. Ehrhardt. 1988. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250.Electrophoresis,9, 255–262.
Ornitz, D.M. and N. Itoh. 2001. Fibroblast growth factors. Genome Biol.,2, 1–12.
Toussaint, M.W., M.J. Wolfe, D.T. Burton, F.J. Hoffmann, T.R. Shedd, and H.S. Gardner. 1999. Histopathology of Japanese medaka (Oryzias latipes) chronically exposed to a complex environmental mixture.Toxicol. Pathol.,27, 652–663.
Yim, U.H., S.H. Hong, W.J. Shim, J.R. Oh, and M. Chang. 2005. Spatio-temporal distribution and characteristics of PAHs in sediments from Masan Bay, Korea.Mar. Pollut. Bull.,50, 319–326.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, S.W., Kim, S.M., Lee, D.K. et al. Characterization of proteins in the muscle oflimanda yokohamae from the masan bay, Korea. Ocean Sci. J. 42, 129–134 (2007). https://doi.org/10.1007/BF03020880
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03020880