Abstract
Purpose
To investigate whether free radical metabolism is changed due to isoflurane treatment and, if so, to elucidate the role of changed free radical metabolism in the nephrotoxicity.
Materials and methods
Fifteen guinea pigs were used in the study. Five were treated with isoflurane in oxygen, five with oxygen and five were controls. Animals were exposed to isoflurane and oxygen three times. Each treatment was performed for 30 min once a day for three consecutive days. Activities of free radical enzymes, Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px); values of antioxidant parameters, antioxidant potential (AOP), non-enzymatic Superoxide radical scavenger activity (NSSA) and oxidation resistance (OR) and, level of an oxidant parameter namely, malondialdehyde (MDA) were determined in the renal tissues of the groups. Blood was also obtained for serum creatinine and urea analyses.
Results
AOP, NSSA, SOD and CAT activities were decreased; (0.0188 ± 0.0026 vs 0.0156 ± 0.0015,P < 0.025; 8.72 ± 1.80vs 6.40 ± 1.22,P < 0.05; 76.71 ± 18.54vs 52.79 ± 1 1.68,P < 0.025; 71.26 ± 15.58vs 55.39 ± 8.83;P < 0.05, respectively) but, MDA level, OR value and GSH-Px activities increased (10.89 ± 1.57 vs 1 5.87 ± 2.97,P < 0.0 1; 0.84 ± 0.34vs 2.28 ± 1.39,P < 0.05; 1.45 ± 0.83 vs 3.45 ± 1.20,P < 0.01, respectively) in kidney tissues from isoflurane-treated group compared with controls. No differences were observed between control and oxygen groups with regard to all analysis parameters except GSH-Px.
Conclusion
Isoflurane impairs the antioxidant defence system and this oxidant stress may play a part in the isoflurane-induced renal toxicity.
Résumé
Objectif
Vérifier si le métabolisme des radicaux libres est changé par l’usage d’isoflurane et, si c’est le cas, préciser le rôle de ce métabolisme transformé sur la néphrotoxicité.
Méthode
L’étude a porté sur quinze cobayes dont cinq ont reçu de l’isoflurane dans de l’oxygène, cinq, de l’oxygène et cinq ont servi de témoins. Les animaux ont été exposés trois fois à l’isoflurane et à l’oxygène. Chaque traitement a été réalisé pendant 30 min, une fois par jour, trois jours consécutifs. On a déterminé dans les tissus rénaux des cobayes: les activités des enzymes des radicaux libres, la superoxyde-dismutase (SOD), la catalase (CAT) et la glutathion-peroxydase (GSH-Px); les valeurs des paramètres antioxydants, le potentiel antioxydant (PAO), l’activité non enzymatique des piégeurs de radicaux superoxydes (ANPS) et la résistance à l’oxydation (RO) ainsi que le niveau d’un paramètre oxydant, à savoir, le malondialdéhyde (MDA). On a aussi prélevé du sang aux fins d’analyses de la créatinine et de l’urée sériques.
Résultats
Les activités des PAO, ANPS, SOD et CAT étaient diminuées (0,0188 ± 0,0026vs 0,0156 ± 0,0015,P < 0,025; 8,72 ± 1,80vs 6,40 ± 1,22,P < 0,05; 76,71 ± 18,54vs 52,79 ± I 1,68,P < 0,025; 71,26 ± 15,58 vs 55,39 ± 8,83;P < 0,05, respectivement) mais le niveau de MDA, la valeur de la RO et les activités de la GSH- Px augmentés (10,89 ± 1,57vs 15,87 ± 2,97,P < 0,01; 0,84 ± 0,34vs 2,28 ± 1,39,P < 0,05; 1,45 ± 0,83vs 3,45 ± 1,20,P < 0,01 respectivement) dans les tissus rénaux du groupe traité à l’isoflurane comparé au groupe témoin. Aucune différence n’a été relevée entre le groupe témoin et celui qui a reçu de l’oxygène quant aux analyses de tous les paramètres, sauf la GSH-Px.
Conclusion
L’isoflurane nuit au système de défense antioxydant et ce stress oxydant peut faire partie de la toxicité rénale induite par l’isoflurane.
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Durak, I., Öztürk, H.S., Dikmen, B. et al. Isoflurane impairs antioxidant defence system in guinea pig kidney. Can J Anesth 46, 797–802 (1999). https://doi.org/10.1007/BF03013919
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DOI: https://doi.org/10.1007/BF03013919