Abstract
In a previous study, we demonstrated that halothane and isoflurane inhibit binding of thromboxane A2 to its receptors on human platelets and thus inhibit prostanoid-induced aggregation strongly. The aim of this study was to determine whether volatile anaesthetics inhibit prostanoid-induced vasoconstriction preferentially. Rat isolated aortic rings were mounted in organ baths and their isometric tension was measured. They were contracted with STA2 (a stable thromboxane A2 analogue), prostaglandin F2α (PGF2α), phenylephrine, and 20 mM KCl, and then exposed to halothane (0.5–3%), isoflurane (0.5–3%), and sodium nitroprusside (SNP, 10−9−3 × 10−7 M). Halothane (2–3%) and isoflurane (2–3%) induced greater relaxation of aortic rings precontracted with STA2 and PGF2α than of those precontracted with phenylephrine (P < 0.01). Halothane induced greater relaxation of rings precontracted with KCl than phenylephrine only at 3%, whereas isoflurance relaxed rings precontracted with KCl more than those with phenylephrine at 0.5, 2 and 3% (P < 0.05). In contrast, SNP relaxed rings precontracted with PGF2α, KCl and phenylephrine equally, but induced smaller relaxations of those precontracted with STA2 (P < 0.05). We conclude that halothane and isoflurane inhibit prostanoid-induced vasoconstriction preferentially, possibly by interacting with prostanoid receptors.
Résumé
Lors d’une étude antérieure, nous avons démontré que l’halothane et l’isoflurane inhibaient la liaison de la thomboxane A2 avec ses récepteurs situés sur les plaquettes humaines et inhibaient fortement ainsi l’agrégation induite par les prostanoïdes. Cette étude vise à déterminer si les anesthésiques volatils inhibent la vasoconstriction induite par les prostanoïdes de façon préférentielle. Des anneaux isolés d’aorte de rat sont introduits dans des bains organiques et leur tension isométrique est mesurée. On les fait a’abord contracter avec du STA2 (un analogue stable de la thomboxane A2), de la prostaglandine F2α (PGF2α), de la phényléphrine, et 20 mM de KCl, et on les expose ensuite à l’halothane (0,5% à 3,0%), l’isoflurane (0,5%–0,3%) et au nitroprussiate de soude (SNP, 10−9−3 × 10−7 M). Vhalothane (2–3%) et l’isoflurane (2–3%) produisent une plus grande relaxation des anneaux aortiques précontractés avec la phényléphrine (P < 0,01). L’halothane produit, mais seulement à 3%, te plus grande relaxation des anneaux précontractés avec le KCL qu’avec la phényléphrine, alors que l’isoflurane aux concentrations de 0,5 2 et 3% relaxe les anneaux précontractés avec le KCl plus que ceux contractés avec la phényléphrine (P < 0,05). Par contre, le SNP relaxe également les anneaux précontractés avec la PGF2α, le KCl et la phényléphrine, mais produit une relaxation moins grande sur ceux qui ont été précontractés avec du STA2 (P < 0,05). Nous concluons que lhalothane et l’isoflurane inhibent la vasoconstriction induite par les prostanoïdes de façon préférentielle, possiblement par interaction avec les récepteurs prostanoïdes.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Sill JC, Ozhan M, Nelson R, Uhl C. Isoflurane-, halothane-, and agonist-evoked responses in pig coronary arteries and vascular smooth muscle cells.In: Blanck TJJ, Wheeler DM (Eds.). Mechanism of Anesthetic Action in Skeletal, Cardiac, and Smooth Muscle. New York: Plenum Press, 1991: 257–69.
Larach DR, Schuler HG, Derr JA, Larach MG, Haensley FA Jr, Zelis R. Halothane selectively attenuates a2-adrenoceptor mediated vasoconstriction,in vivo andin vitro. Anesthesiology 1987; 66: 781–91.
Hirakata H, Hatano Y, Ushikubi F, Narumiya S, Mori K. Effect of inhalation anesthetics on platelet aggregation-reduction of ligand-binding affinity of platelet thromboxane A2 receptor by halothane. Anesthesiology 1992; 77: A434.
Hirata M, Hayashi Y, Ushikubi F, et al. Cloning and expression of cDNA for a human thromboxane A2 receptor. Nature 1991; 349: 617–20.
Hatano Y, Nakamura K, Yakushiji T, Nishiwada M, Mori K. Comparison of the direct effects of halothane and isoflurane on large and small coronary arteries isolated from dogs. Anesthesiology 1990; 73: 513–7.
Karaki H, Weiss GB. Calcium release in smooth muscle. Life Sci 1988; 111–22.
Nishimura J, van Breemen C. Direct regulation of smooth muscle contractile elements by second messengers. Biochem Biophys Res Commun 1989; 163: 929–35.
Somlyo AV, Bond M, Somlyo AP, Scarpa A. Inositol triphosphate-induced calcium release and contraction in vascular smooth muscle. Proc Natl Acad Sci U S A 1985; 1985: 5231–35.
Fukuo K, Morimoto S, Koh E, et al. Effect of prostaglandins on the cytosolic free calcium concentration in vascular smooth muscle cells. Biochem Biophys Res Commun 1986; 136: 247–52.
Murad F. Cyclic guanosine monophosphate as a mediator of vasodilation. J Clin Invest 1986; 78: 1–5.
Harioka T, Hatano Y, Mori K, Toda N. Trimethaphan is a direct arterial vasodilator and an α-adrenoceptor antagonist. Anesth Analg 1984; 63: 290–6.
Bollen BA, Tinker JH, Harmsmeyer K. Halothane relaxes previously constricted isolated porcine coronary artery segments more than isoflurane. Anesthesiology 1987; 66: 748–52.
Ogletree ML. Overview of physiological and pathophysiological effects of thromboxane A2. Federation Proceedings 1987; 46: 133–8.
Behrman HR, Romero RJ. Prostaglandins and prostragladin-like products in reproduction: eicosanoids, peroxides, and oxygen radicals.In: Yen SSC, Jaffe RB (Eds.). Reproductive Endoclinology, 3rd ed., Philadelphia: W.B. Saunders Company, 1991: 238–72.
Munson ES, Embro WJ. Enflurane, isoflurane, and halothane and isolated human uterine muscle. Anesthesiology 1977; 46: 11–4.
Dalsgaard-Neiben J, Risbo A, Simmelkjaer P, Gormsen J. Impaired platelet aggregation and increased bleeding time during general anaesthesia with halothane. Br J Anaesth 1981; 53: 1039–41.
Pavlin EG, Su JY. Cardiopulmonary pharmacology.In: Miller RD (Ed.). Anesthesia, 3rd ed., New York: Churchill Livingstone Inc., 1990: 105–10.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamamoto, M., Hatano, Y., Kakuyama, M. et al. Halothane and isoflurane preferentially inhibit prostanoid-induced vasoconstriction of rat aorta. Can J Anaesth 41, 991–995 (1994). https://doi.org/10.1007/BF03010943
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03010943