Abstract
Anaesthesia and surgery are known to depress granulocyte function in the early postoperative period, leading to deterioration of the immune defence against infection. Carbohydratelectin interactions may play an important role in the activities of phagocytic cells in that they facilitate initial host defence in the event of microbial antigenic challenge. A panel of biotinylated (neo)glycoproteins (chemically glycosilated carrier proteins) was used to detect endogenous carbohydrate-binding receptors /lectins/, on peripheral blood polymorphonuclear leukocytes of patients undergoing prolonged anaesthesia for replantation surgery. Four hours after induction of anaesthesia, a progressive decline of expression of endogenous sugar receptors on granulocytes was detected using the labelled (neo)glycoproteins lactose-BSA, N-acetyl-D-glucosamine-BSA, D-mannose-BSA, sialic-acid-BSA and D-xylose-BSA. Concomitant changes in peripheral white blood cell counts and the lack of depression in the absence of general anaesthetic agents suggested the existence of a possible relationship between reduced expression of (neo)glycoprotein receptors to impaired granulocyte function and anaesthetic-induced immunodepression.
Résumé
Nous savons que l’anesthésie et la chirurgie dépriment la fonction des granulocytes dans la période postopératoire immédiate, provoquant une détérioration de la défence immunitaire contre l’infection. Les interactions hydrate de carbone-lectine pourraient jouer un rôle important dans les activités des cellules phagocytaires en façilitant la défense initiale de l’hôte lors d’une confrontation avec des antigènes microbiaux. Un panneau de (néo) glycoprotéines liées à la biotine (protéines porteuses glycosilées chimiquement) a été utilisé pour détecter les récepteurs endogènes se liant aux hydrates de carbone, les lectines, dans les leucocytes polymorphonucléaires du sang périphérique de patients subissant une anesthésie prolongée dans le cadre d’une chirurgie de réimplantation. Quatre heures après l’induction de l’anesthésie, une diminution progressive de l’expression des récepteurs endogènes de sucre sur les granulocytes a été détectée en utilisant les (néo) glycoprotéines marquées lactose-BSA, N-acétyl-D-glucosamine-BSA, D-mannose-BSA, acide sialique-BSA et D-xylose-BSA. Les changements concomitants dans les décomptes des globules blancs périphériques et le manque de dépression en l’absence d’agents anesthésiques généraux ont suggéré l’existence d’une relation possible entre une expression diminuée des récepteurs des (néo) glycoprotéines et la fonction granulocitaire altérée, et l’immunodépression induite par l’anesthésie.
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References
Bruce DL, Koepke JA. Changes in granulopoiesis in the rat associated with prolonged halothane anesthesia. Anesthesiology 1966; 27: 811–6.
Nunn JF, O’Morain C. Nitrous oxide decreases motility of human neutrophils in vitro. Anesthesiology 1982; 56: 45–8.
Bardosi L, Tekeres M. Impaired metabolic activity of phagocytic cells after anaesthesia and surgery. Br J Anaesth 1985; 57: 520–3.
Brodsky JB, Baden JM, Serra M et al. Nitrous oxide inactivates methionin synthetase activity in rat testis. Anesthesiology 1984; 61: 66–8.
Sharon N. Carbohydrates as recognition determinants in phagocytosis and lectin-mediated killing of target cells. Biol Cell 1984; 51: 239–46.
Clohisy DR, Bar-Sharit Z, Chappel JC et al. 1,25-Dihydroxivitamin D3 modulates bone marrow macrophage precursor proliferation and differentiation. J Biol Chem 1987; 262: 15922–9.
Stoolman LM, Tenforde TS, Rose SD. Phosphomannosyl receptors may participate in the adhesive interaction between lymphocytes and high endothelial venules. J Cell Biol 1984; 99: 1535–40.
Matsuoka T, Tavassoli M. Purification and partial characterisation of membrane-homing receptors of two cloned murine hemopoietic progenitor cell line. J Biol Chem 1989; 264: 20193–8.
Gabius H-J, Bardosi A. Neoglycoproteins as tools in glycohistochemistry. Prog Histochem Cytochem 1991; 22: 3–19.
Bardosi A, Dimitri T, Gabius H-J. (Neo)glycoproteins as tools in neuropathology: histochemical patterns of the extent of expression of endogenous carbohydrate-binding receptors, like lectins, in meningeomas. Virchows Arch (B) 1988; 65: 35–43.
Gabius H-J, Bodanowitz S, Schauer A. Endogenous sugar-binding receptors in human breast tissue and benign and malignant breast lesions. Cancer 1988; 61: 1125–31.
Gabius H-J, Kohnke B, Hellmann T et al. Comparative histochemical and biochemical analysis of endogenous receptors for glycoconjugates in human and pig nerve. J Neurochem 1988; 51: 756–65.
Bardosi A, Dimitri T, Behrends T et al. Is part of the molecular basis of the perineurial barrier function the lack of endogenous carbohydrate-binding proteins. J Neurosci Res 1989; 22: 65–73.
Koenig A, Koenig UD, Stoeckel H. Anästhesie und Immunologie. Eine übersicht. Pract Anästh 1978; 13: 415–29.
Hole A. Depression of monocytes and lymphocytes by stress-related humoral factors and anaesthetic related drugs. Acta Anaesth Scand 1984; 28: 280–6.
Walton B. Anaesthesia, surgery and immunology. Anaesthesia 1978; 33: 322–8.
Welch WD. Halothane reversibly inhibits human neutrophil bacterial killing. Anesthesiology 1981; 55: 650–4.
Moudgil GC, Pandya AR, Ludlow DJ. Influence of anaesthesia and surgery on neutrophil chemotaxis. Can Anaesth Soc J 1981; 28: 232–8.
Moudgil GC, Allan RB, Russell RJ et al. Inhibition, by anaesthetic agents, of human leukocyte locomotion towards chemical attractants. Br J Anaesth 1977; 49: 97–105.
Vandenbroucke-Grauls CMJE, Thijessen HMWM, Verhoef J. Interaction between human polymorphonuclear leukocytes and Staphylococcus aureus in the presence and absence of opsonins. Immunology 1984; 52: 427–31.
Ofek I, Sharon N. Lectinophagocytosis: a molecular mechanism of recognition between cell surface sugars and lectins in the phagocytosis of bacteria. Infect Immun 1988; 56: 539–47.
Harlan JM, Killen PD, Senecal FM et al. The role of neutrophil membrane glycoprotein GP-150 in neutrophil adherence to endothelium in vivo. Blood 1985; 66: 167–78.
Beatty PG, Harlan JM, Rosen H et al. Absence of monoclonal antibody defined protein complex in boy with abnormal leukocyte function. Lancet 1984; i: 535–7.
Gabius H-J. Endogenous lectins in tumors and the immune system. Cancer Invest 1987; 5: 39–46.
Kieda CMT, Bowles DJ, Ravid A, Sharon N. Lectins in lymphocyte membranes. FEBS Lett 1978; 94: 391–6.
Gabius H-J, Nagel GA. (Eds.), Lectins and Glycoconjugates in Oncology. New York: Springer Verlag, 1988.
Czop JK. The role of β-glucan receptors on blood and tissue leukocytes in phagocytosis and metabolic activation. Pathol Immun Res 1986; 5: 286–96.
Rottini G, Cian F, Soranzo R et al. Evidence for the involvement of human polymorphonuclear leukocyte mannose-like receptors in the phagocytosis of Escherichia coli. FEBS Lett 1979; 105: 307–12.
Silverblatt FJ, Dreyer JS, Schauer S. Effect of pili on susceptibility of Escherichia coli to phagocytosis. Infect Immun 1979; 34: 218–23.
Stahl PD, Rodman JS, Miller MJ et al. Evidence for receptor-mediated binding of glycoproteins, glycoconjugates and lysosomal glycosidases by alveolar macro-phages. Proc Natl Acad Sci USA 1978; 75: 1399–403.
Sepherd VL, Lee YC, Schlesinger PH et al. L-fucose terminated glycoconjugates are recognized by pinocytosis receptors on macrophages. Proc Natl Acad Sci USA 1981; 78: 1019–22.
Bardosi L, Bardosi A, Hendrys M et al. Reduced expression of mannose-specific receptors on murine peripheral blood polymorphonuclear leukocytes following prolonged anaesthesia with different inhalation agents. Acta Anaesthesiol Scand 1990; 34: 286–90.
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Supported by a fellowship from the Max-Planck-Society.
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Bardosi, L., Bardosi, A. & Gabius, HJ. Changes of expression of endogenous sugar receptors by polymorphonuclear leukocytes after prolonged anaesthesia and surgery. Can J Anaesth 39, 143–150 (1992). https://doi.org/10.1007/BF03008645
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DOI: https://doi.org/10.1007/BF03008645