Summary
We have perfused malignant hyperthermia susceptible and normal isolated pig livers with halothane for one hour. The liver temperatures, oxygen and carbon dioxide tensions, the base deficits and lactate concentrations in blood entering and leaving the liver have been measured at the beginning and at the end of the perfusion. Statistical analysis has shown that there are no significant differences in these parameters between the beginning and the end of the perfusion period or between the normal and the malignant hyperthermia susceptible livers. We conclude, therefore, that the livers of malignant hyperthermia susceptible pigs are either normal or else, if abnormal, the abnormalities are sufficiently benign as to be not measurably expressed.
Résumé
Des foies isolés de porcs susceptibles à ľhyperthermie maligne ont été perfusés à ľhalothane pendant une heure. Les températures du foie, les tensions de ľoxygène et du gaz carbonique, le déficit en bases, et la concentration de lactate ont été mesurés dans le sang en amont et en aval de ľorgane au début et à la fin de la perfusion. Ľanalyse statistique n’a pas révélé de différence significative dans ces paramètres entre le début et la fin de la perfusion ou entre les foies normaux et les foies susceptibles à ľhyperthermie maligne. De là, nous concluons que le foie du porc susceptible à ľhyperthermie maligne est possiblement normal. S’il ne ľest pas, cette anomalie pourrait-être telle qu’on ne puisse la quantifier.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Brut, B.A. Malignant hyperthermia — 1977.In: Complications in Anesthesiology (eds. Orkin, F.K. and Cooperman, L.H.), J.B. Lippincott Co., Philadelphia, Pa. In press (1977).
Brut, B.A. Malignant hyperthermia — aetiology and pathophysiology.In: Anaesthesiology, Proceedings of the VI World Congress of Anaesthesiology, Mexico City, April 24-30, 1976. Ed. Husxz, E. and Lunn, J.N. Excerpta Medica, Amsterdam-Oxford (1977).
Berman, M.C. &Kench, J.E. Biochemical features of malignant hyperthermia in Landrace pigs.In: International Symposium on Malignant Hyperthermia (ed. Gordon, Britt, Kalow), Charles C. Thomas, Springfield, p. 287 (1973).
Brucker, R.F., Williams, C.H., Popinigis, J., Galvez, T.L., Vail, W.J., &Taylor, C.A.In vitro studies on liver mitochondria and skeletal muscle sarcoplasmic reticulum fragments isolated from hyperpyrexic swine.In: International Symposium on Malignant Hyperthermia (ed. Gordon, Britt, Kalow), Charles C. Thomas, Springfield, p. 238 (1973).
Rouiller, Ch. (ed.) The Liver — Morphology, Biochemistry, Physiology. Volumes I and II. Academic Press, N.Y. (1963).
Sandow, A. Skeletal muscle. Ann. Rev. Physiol.32: 1040 (1970).
Hoyle, G. How is muscle turned on and off? Sci. Am.222: 85 (1970).
Hasselbach, W. Relaxing factor and the relaxation of muscle. Progress in Biophysics14: 1 (1964).
Huxley, H.E. Structural evidence concerning the mechanism of contraction in striated muscle.In: Muscle (ed. Paul, W.M., Daniel, E.E., Kay, C.M., and Monckton, G.), Pergamon Press, Oxford (1965).
Porter, K.R. &Franzini-Armstrong, C. The sarcoplasmic reticulum. Sci. Am.212: 73 (1965).
Jones, E.W. &Nelson, T.E. Personal communication. Oklahoma State University, Stillwater, Oklahoma.
Britt, B.A., Kalow, W., Gordon, A., Humphrey, J.G., &Rewcastle, N.B. Malignant hyperthermia — an investigation of five patients. Canad. Anaesth. Soc. J.20: 431 (1973).
Britt, B.A., Endrenyi, L., Kalow, W., &Peters, P.L. The adenosine triphosphate (ATP) depletion test: comparison with the caffeine contracture test as a method of diagnosing malignant hyperthermia susceptibility. Canad. Anaesth. Soc. J.23: 624 (1976).
Huckell, V.F.,Staniloff, H.M.,McLaughlin, P.R.,Britt, B.A., &Morch, J.E. Cardiovascular manifestations of normothermic malignant hyperthermia. Presented at the 2nd International Symposium on Malignant Hyperthermia, Denver, Colo., April 1–3, 1977.
Huckell, V.F.,Staniloff, H.M.,Britt, B.A.,Waxman, M.B.,Feiglin, D.H.,McLaughlin, P.R., &Morch, J.E. Thallium-201 myocardial imaging in malignant hyperthermia: a membrane disease. Accepted for presentation at the American College of Cardiology, March 6–9, 1978, Anaheim, California.
Huckell, V.,Staniloff, H.,Britt, B.A.,Feiglin, D.,McLaughlin, P.,Waxman, M., &Morch, J. Cardiac abnormalities in malignant hyperthermia. Accepted for presentation at the 50th Scientific Sessions, American Heart Association, November 28–December 1, 1977, Miami, Fla.
Harrison, J.E., McNeill, K.G., Meema, H.E., Fenton, S., Oreopoulas, D.G., &Sturtridge, W.C. Partial-body calcium measurements byin vivo neutron activation analysis: comparisons with X-ray photodensitometry measurements of the radius. J. Nucl. Med.15: 929 (1974).
Harrison, J.E. 1VNAA experience with idiopathic osteoporosis and with osteoporosis secondary to hyperthyroidism and malignant hyperthermia. Presented at the 2nd Conference onIn Vivo Neutron Activation Analysis, April 5–10, 1976, Glasgow, Scotland.
Harrison, J.E.,Sturtridge, W.C.,Williams, C.,Watts, J., &McNeill, K.G. A bone calcium index based on partial body calcium measurements byin vivo activation analysis. J. Nucl. Med.16: (1975).
Puszkin, E., Puszkin, S., Lo, L.W., &Tanenbaum, S.W. Binding of cytochalasin D to platelet and muscle myosin. J. Biol. Chem.248: 7754 (1973).
Johnson, S.A., van Horn, D.L., Pederson, H.J., &Marr, J. The function of platelets: a review. Transfusion6: 3 (1966).
Puszkin, S., Puszkin, E., Katz, A.M., &Aledort, L.M. Control of platelet actomyosin activity: effect of ADP on superprecipitation and ATPase activity of human platelet actomyosin. Biochim. Biophys. Acta347: 102 (1974).
Lebowitz, E.A. &Cooke, R. Contractile properties of myosin from human blood platelets. Circulation56: Abstract #284 (1977).
Puszkin, E.G.,Spaet, T.H.,Maimon, J., &Puszkin, S. Platelet a-actinin: effect on actin properties. Circulation56: Abstract #285 (1977).
Keppens, S., Vanderheede, J.R., &de Wulf, H. On the role of calcium as second messenger in liver for the hormonally induced activation of glycogen phosphorylase. Biochim. Biophys. Acta496: 448 (1977).
Friedmann, N. &Park, C.R. Early effects of 3′,5′-adenosine monophosphate on the fluxes of calcium and potassium in the perfused liver of normal and adrenalectomized rats. Proc. Nat. Acad. Sci.61: 504 (1968).
Lehninger, A.L. Mitochondria and calcium ion transport. Biochem. J.119: 129 (1970).
Drahota, Z., Carafoli, E., Rossi, C.S., Gamble, R.L., &Lehninger, A.L. The steady state maintenance of accumulated Ca++ in rat liver mitochondria. J. Biol. Chem.240: 2712 (1965).
Borle, A.B. Cyclic AMP stimulation of calcium efflux from kidney, liver, and heart mitochondria. J. Membrane Biol.16: 221 (1974).
Britt, B.A., Endrenyi, L., Peters, P.L., Kwong, F.H.-F., &Kadijevic, L. Screening of malignant hyperthermic susceptible families by CPK measurement and other clinical investigations. Canad. Anaesth. Soc. J.23: 263 (1976).
McIntosh, D. &Berman, M.C. Neutral lipid and phospholipid composition of normal and myopathic skeletal muscle of pigs. S. African Med. J.48: 1221 (1974).
Moulds, R.F.W. &Denborough, M.A. Biochemical basis of malignant hyperpyrexia. Brit. Med. J.2: 241 (1974).
Nelson, T.E., Bedell, D.M., &Jones, E.W. Porcine malignant hyperthermia. Anesthesiology42: 301 (1975).
Nelson, T.E.,Jones, E.W., &Holbert, D. Malignant hyperthermia: sarcolemmal defect? Unpublished manuscript.
Heffron, J.J.A.,Theye, R.A., &Gronert, G.A. Porcine malignant hyperthermia: calcium binding and release by isolated sarcoplasmic reticulum. Presented at the 2nd International Symposium on Malignant Hyperthermia, Denver, Colo., April 1–3,1977.
Cheah, K.S. &Cheah, A.M. The trigger for PSE condition in stress susceptible animals. J. Sci. Fd. Agric.27: 1137 (1976).
Denborough, M.A.,Forster, J.F.A.,Hudson, M.C.,Carter, N.G., &Zapf, P. Malignant hyperpyrexia-a serious, preventable complication of general anaesthesia. Australasian Congress of Anaesthesiology, Melbourne, Australia, 1970.
Britt, B.A. Unpublished data, 1977.
Gordon, R.A., Britt, B.A., &Kalow, W. (eds.). International Symposium on Malignant Hyperthermia, Charles C. Thomas, Springfield (1973).
Author information
Authors and Affiliations
Additional information
This work was supported by a grant from the Medical Research Council of Canada.
Rights and permissions
About this article
Cite this article
Brut, B.A., Shandling, B., Endrenyi, L. et al. Perfusion of malignant hyperthermia susceptible and normal isolated pig livers with halothane. Canad. Anaesth. Soc. J. 25, 373–379 (1978). https://doi.org/10.1007/BF03006566
Issue Date:
DOI: https://doi.org/10.1007/BF03006566