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Diltiazem and nifedipine reduce thein vitro contracture response to halothane in malignant hyperthermia-susceptible muscle

Canadian Journal of Anaesthesia volume 37, Article number: 556 (1990) Cite this article

Abstract

The effects of diltiazem (1 μM) and nifedipine (I μM) were examined separately on the in vitrohalothane tests for malignant hyperthermia (MH) susceptibility. Eighteen patients with MH susceptibility were diagnosed as MH-susceptible (MHS) according to the protocol of the European MH Group. In addition, halothane tests were carried out in the presence of either diltiazem (ten patients) or nifedipine (eight patients). These two calcium channel blockers significantly reduced the halothane contracture. Furthermore, in five of the ten MHS patients tested in the presence of diltiazem as well as in five of the eight MHS patients tested in the presence of nifedipine the halothane contracture test could be classified as negative. It is concluded that the presence of clinical concentrations of either diltiazem or nifedipine in the muscle bath affects the in vitrodiscrimination for MH susceptibility to halothane.

Résumé

Les effets du diltiazem (1 μM) et de la nifedipine (1 μM) ont été étudiés separement sur le test halothane in vitrode depistage de l’hyperthermie maligne (HM). Des lambeaux musculaires provenant de dix huit patients sensibles à l’HM ont été testes suivant le protocole del’« European MH Group ». Le test à l’halothane à été realise seul, puis en présence de diltiazem (dix patients) ou de nifédipine (huit patients). Le pretraitement des lambeaux musculaires au diltiazem classe cinq des dix sujets susceptibles comme negatif à l’halothane. De meme cinq des huit lambeaux musculaires pretraités à la nifedipine classe ces sujets suceptibles comme negatif au test à i halothane. Il semble done que la présence de concentrations cliniques de diltiazem ou de nifedipine dans le liquide de perfusion affecte les resultats du test à l’halothane.

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Affiliations

  1. Department of Anesthesiology, University of Lille, Lille, France

    Pascal J. Adnet, Renee M. Krivosic-Horber, Ghislain Haudecoeur, Hugo G. Reyford, Monique M. Adamantidis & Bernard A. Dupuis

  2. Department of Pharmacology, University of Lille, Lille, France

    Pascal J. Adnet, Renee M. Krivosic-Horber, Ghislain Haudecoeur, Hugo G. Reyford, Monique M. Adamantidis & Bernard A. Dupuis

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  1. Pascal J. Adnet
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  2. Renee M. Krivosic-Horber
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  3. Ghislain Haudecoeur
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  4. Hugo G. Reyford
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  5. Monique M. Adamantidis
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  6. Bernard A. Dupuis
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Supported by l’Association Françhise des Myopathcs.

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Adnet, P.J., Krivosic-Horber, R.M., Haudecoeur, G. et al. Diltiazem and nifedipine reduce thein vitro contracture response to halothane in malignant hyperthermia-susceptible muscle. Can J Anaesth 37, 556 (1990). https://doi.org/10.1007/BF03006325

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  • DOI: https://doi.org/10.1007/BF03006325

Key words

  • anaesthetics, volatile: halothane
  • hyperthermia: malignant
  • pharmacology: calcium channel blockers, diltiazem, nifedipine