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An asymmetric distribution of dcm-inhibiting restriction sites (dcm-sites) takes place in 10 human genes with methylation-dependent regulation. These genes are dcm-site enriched upstream and dcm-site poor downstream. Along them, there is a scattering of hypermethylated introns and hypomethylated exons with a common code: the CpG dinucleotides characterize promoters; Gp0Cs characterize introns; TpCs and CpOCs are in small concentrations in exons. Housekeeping (HK) genes contain more dcm-sites when compared with tissuespecific (TS) genes. This depends on the higher number of dcm-sites in their promoters and introns. In exons, the relatively lower number of dcm-sites is almost the same in both HKs and TSs. Going upstream-downstream, the absolute number of dcm-sites as well as the frequence of these sites per nucteotide units decrease in introns and increase in exons. This difference is highly discriminated for TSs and less discriminated for HKs.
In 10 geni umani con controllo metilazione-dipendente ha luogo una distribuzione asimmetrica dei dcm-siti inibitori di restrittasi. Questi geni sono ricchi di dcm-siti verso l’estremit 5’ e poveri di dcm-siti verso l’estremitä 3’. Nel loro ambito c’é un’intermittenza di introni ipermetilati ed esoni ipometilati con codice comune: i dinucleotidi CpG caratterizzano i promotori; quelli GpC caratterizzano gli introni; i TpC e i CpC si trovano in piccole concentrazioni negli esoni. I geni housekeeping (HK) hanno più dcm-siti rispetto ai tessutospecifici (TS). Ciò dipende dal numero più alto di dcm-siti nei loro promotori e intronL Negli esoni, il numero relativamente pi basso di dcm-siti è quasi identico negli HK e nei TS. In direzione 5’-3’, sia il numero assoluto di dcm-siti che la frequenza di questi siti per unitä di nucleotide decrescono negli introni e aumentano negli esoni. Tale differenza ä altamente discriminata per i TS e meno discriminata per gli HK.
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Nella seduta del 24 aprile 1992.
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di VolPE, N., Iacovacci, P., Esvosrro, C. et al. Common language in eukaryotic genes with methylationdependent regulation. Rend. Fis. Acc. Lincei 3, 383–394 (1992). https://doi.org/10.1007/BF03002945
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DOI: https://doi.org/10.1007/BF03002945