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Determination of the sequence and study of the expression of a gene for the heat shock protein (hsp) 70 of the sea urchin Paracentrotus lividus

Determinazione delta sequenza nucleotidica e studio dell’espressione di un gene per la proteina da shock da calore di 70 kDa del riccio di mare Paracentrotus lividus

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Abstract

The sequence of a gene coding for the hsp70 isolated from a P.lividus genomic library is reported. This gene is interrupted by a long intron at 183 nt downstream the translation start codon. Its promoter region contains three heat shock elements, thus indicating that the gene is an inducible one. Northern blot analysis shows that this gene is transcribed following heat shock and that its transcription products bind to ribosomes.

Riassunto

Si riporta la sequenza nucleotidica di un gene per la proteina da shock da calore di 70 kDa isolato da una genoteca di riccio di mareParacentrotus lividus. Quesro gene è interrotto da un lungo introne a 183 nucleotidi a valle della tripletta per l’inizio della traduzione. La sua regione del promotore contiene tre elementiheat shock, il ehe indica ehe trattasi di un gene inducibile. Un’analisi perNorthern blot mostra ehe il gene è trascritto a seguito di shock da calore e che i prodotti della sua trascrizione si legano ai ribosomi.

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Authors and Affiliations

  1. Dipartimento di Biologia Cellulare e dello Sviluppo, Università degli Studi, di Palermo — Via Archirafi, 22 -90123, Palermo

    Maria Grazia Ferraro, Domenico Cascino, Gabriella Sconzo & Giovanni Giudice

  2. Istituto di Biologia dello Sviluppo - C.N.R. - Via Archirati, 20 — 90123, Palermo

    Giuseppe Scardina & Giovanni Giudice

Authors
  1. Maria Grazia Ferraro
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  2. Giuseppe Scardina
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  3. Domenico Cascino
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  4. Gabriella Sconzo
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  5. Giovanni Giudice
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Nella seduta del 24 aprile 1992.

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Ferraro, M.G., Scardina, G., Cascino, D. et al. Determination of the sequence and study of the expression of a gene for the heat shock protein (hsp) 70 of the sea urchin Paracentrotus lividus. Rend. Fis. Acc. Lincei 3, 375–381 (1992). https://doi.org/10.1007/BF03002944

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  • DOI: https://doi.org/10.1007/BF03002944

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