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Mouse chondrocytes in culture: New prospects for the study of bone formation in mammals

Condrociti di topo in cultura: Nuove prospettive per lo studio delta formazione dell’osso nei mammiferi

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Abstract

Isolated chondrocytes from mouse ribs have been put in culture and maintained for several weeks, till confluence. They retain differentiation, and preliminary data of cells characterization and growth are reported. Electron microscope observations of the cellsin vitro grown are superimposable to those performed on chondrocytes derived from live animals. Mouse chondrocytes, grownin vitro, represent a promising system for the study of bone differentiation, growth and regeneration, also considering the several mutations affecting skeleton formation known in mice, like cn/cn (achondroplasia).

Riassunto

Condrociti isolati da costole di topo sono stati messi in coltura e mantenuti per diverse settimane, flno al raggiungimento della confluenza. In questo lavoro sono riportati dati prelirninari sulla loro caratterizzazione, stato di differenziamento e crescita. Le osservazioni al microscopio elettronico delle cellule cresciutein vitro sono sovrapponibili a quelle mostrate dai condrocitiin vivo. Le colture di condrociti di topo rappresentano un metodo utile per lo studio della diffèrenziazione, crescita e rigenerazione dell’osso, anche considerando le numerose mutazioni presenti nel topo che interessano la formazione dello scheletro, quali 1’acondroplasia (cn/cn).

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Authors and Affiliations

  1. Dipartimento di Sanità Pubblica e Biologia Cellulare, II Università degli Studi di Roma«, Tor Vergata» Via O. Raimondo, 8, 00173, Roma

    Gabriella Argentin, Rosadele Cicchetti, Antonella Sarperi, Domenico Cocchia, Benedetto Nicoletti & Socio G. Chieffi

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  1. Gabriella Argentin
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  2. Rosadele Cicchetti
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  3. Antonella Sarperi
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  4. Domenico Cocchia
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  5. Benedetto Nicoletti
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  6. Socio G. Chieffi
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Nella seduta del 12 giugno 1992.

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Argentin, G., Cicchetti, R., Sarperi, A. et al. Mouse chondrocytes in culture: New prospects for the study of bone formation in mammals. Rend. Fis. Acc. Lincei 4, 9–17 (1993). https://doi.org/10.1007/BF03001179

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