Summary and Conclusions
Various oxidized bile salts, dehydrocholic acid (Decholin), ketocholanic acids (Ketochol), oxidized conjugated ox-bile salts (Dechacid No. 14), and oxidized conjugated hog-bile salts (Dechacid No. 22) were administered orally to dogs and rats to determine what “toxic” effects they might have on the functional activity of the liver. Toxic effects were examined for by observing the weight of the animals, by determining bromsulphalein clearance, by analyzing the liver for total fat and glycogen content, and by histological study of sections of the liver and kidney.
The oral administration of 3 and 5 grams of the various oxidized bile preparations daily for 3 to 7 months to dogs and the feeding of 0.3 gm. per kilo daily of these preparations to white rats for at least 1 month resulted in no change in the body weight of the animals, no alteration in the normal bromsulphalein excretion test, and no abnormal changes in the concentrations of total liver fat and glycogen. Histological studies of both the liver and kidney tissues revealed no destructive or degenerative changes in these organs.
Thus, we were unable by the methods used to obtain any evidence indicating that oxidized bile preparations are toxic when given orally in relatively large doses for 3 to 7 months in dogs and for 1 month in rats.
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Aided in part by the E. L. Dawes Research Fund.
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Berman, A.L., Snapp, E., Ivy, A.C. et al. The effect of long-continued ingestion of oxidized bile acids on the dog and rat. Jour. D. D. 7, 280–284 (1940). https://doi.org/10.1007/BF02997324
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DOI: https://doi.org/10.1007/BF02997324