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Hypermutation in stationary-phaseE. coli: tales from thelac operon

  • Special Section: Stationary-Phase Mutations In Microorganisms
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Abstract

Escherichia coli cells are capable of complex regulatory responses to environmental conditions and stresses. In some circumstances, the response includes an increase in the mutation rate, effectively mutagenizing the genome. The classic example is the SOS response to DNA damage. Recent work indicates that other environmental stresses can also result in mutation of the genome. Modulation of mutation rate may be a more prevalent stress response than previously thought. In this review we focus on genome-wide mutation inE. coli cells subjected to a nonlethal genetic selection for reversion of alac frameshift allele. Reversion of thelac frameshift allele occurs via a novel mechanism that requires homologous recombination functions, and is enhanced by transiently diminished postsynthesis mismatch repair. A model for recombination-dependent stationary-phase mutation will be presented and its relevance for genome-wide mutation discussed.

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  1. Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, S809A, 77030-3498, Houston, TX, USA

    Mary-Jane Lombardo & Susan M. Rosenberg

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  1. Mary-Jane Lombardo
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  2. Susan M. Rosenberg
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Lombardo, MJ., Rosenberg, S.M. Hypermutation in stationary-phaseE. coli: tales from thelac operon. J Genet 78, 13–20 (1999). https://doi.org/10.1007/BF02994698

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