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Purification of orthodiphenol oxidoreductase from potatoes by affinity chromatography on coir pith lignin

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Abstract

Orthodiphenol oxidoreductase, ODOR (E.C.I.10.3.1) from potato tubers (Solanurn tuberosum) has been purified on coir pith lignin by affinity chromatography. All isozymes get adsorbed and can be eluted with 70% recovery. The enzyme shows high purity, as judged by specific activity and polyacrylamide gel electrophoresis.

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Author notes

  1. Harjinder Parwana

    Present address: , 2536/Sec 22-C, 160022, Chandigarh

Authors and Affiliations

  1. Department of Chemistry University of Poona Pune, 411 007, India

    Mahabaleshwar V. Hegde, Ashok V. Anvekar, Harjinder Parwana & Anant J. Mukhedkar

Authors
  1. Mahabaleshwar V. Hegde
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  2. Ashok V. Anvekar
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  3. Harjinder Parwana
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  4. Anant J. Mukhedkar
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Hegde, M.V., Anvekar, A.V., Parwana, H. et al. Purification of orthodiphenol oxidoreductase from potatoes by affinity chromatography on coir pith lignin. Journal of Solid Phase Biochemistry 5, 139–145 (1980). https://doi.org/10.1007/BF02994453

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  • DOI: https://doi.org/10.1007/BF02994453

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