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Fluorometric and electrochemical methods for assay of biological and environmental substances

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Abstract

The isolation, purification, and analytical use of enzymes from microbial sources are described: L-lysine decarboxylase fromE. coli, L-methionine ammonia lyase fromPseudomonasovalis, nitrate reductase fromChlorella vulgaris, nitrite reductase fromAzotobacter chroococcum, sulfate reductase fromDesulvibrio desulficans, and creatininase fromPseudomonas. Both electrochemical, fluorometric, and spectrophotometric methods are proposed for the assay of enzyme activity, and specific, sensitive, fast, and inexpensive methods are described for the assay of 10-1-10-1 M concentrations of L-lysine, L-methionine, nitrate, nitrite, creatinine, and triglycerides.

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Authors and Affiliations

  1. Department of Chemistry, University of New Orleans New Orleans, 70122, Louisiana

    George G. Guilbault, S. S. Kuan, R. Kuan, W. Fung, G. Sung, S. Chen & H. Winartasaputra

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  1. George G. Guilbault
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  2. S. S. Kuan
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  3. R. Kuan
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  4. W. Fung
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  5. G. Sung
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  6. S. Chen
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  7. H. Winartasaputra
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Guilbault, G.G., Kuan, S.S., Kuan, R. et al. Fluorometric and electrochemical methods for assay of biological and environmental substances. Journal of Solid Phase Biochemistry 5, 125–138 (1980). https://doi.org/10.1007/BF02994452

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  • DOI: https://doi.org/10.1007/BF02994452

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