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Determination of genetic differences between fluid and nonfluid variants ofClavibacter michiganensis subsp.sepedonicus using rep-PCR technique

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Abstract

Testing of 23 isolates ofClavibacter michiganensis subsp.sepedonicus for analysis by rep-PCR (using BOX, ERIC, REP primer sets) was used for the purpose of localization of genetic markers for fluid and/or nonfluid strains. None of the primer sets was successful in detecting genetic differences between the isolates and no polymorphism was generated.

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Authors and Affiliations

  1. Department of Plant Virology, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, 370 05, Ceské Budějovice, Czechia

    J. Fousek & I. Mráz

  2. Faculty of Biological Sciences, University of South Bohemia, 370 05, České Budějovice, Czechia

    J. Fousek

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  1. J. Fousek
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  2. I. Mráz
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Correspondence to J. Fousek.

Additional information

This work was supported by theGrant Agency of the Czech Republic (grant no. GA 522/00/0887) and by theGrant Agency of the Academy of Sciences of the Czech Republic (grant no. S 5051014).

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Fousek, J., Mráz, I. Determination of genetic differences between fluid and nonfluid variants ofClavibacter michiganensis subsp.sepedonicus using rep-PCR technique. Folia Microbiol 48, 682–686 (2003). https://doi.org/10.1007/BF02993479

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  • DOI: https://doi.org/10.1007/BF02993479

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