Abstract
Recombinant haploid segregants were recovered in filamentous fungusAspergillus nidulans (Eidam)G. Winter directly from the heterokaryons instead of diploid segregants (process described earlier as parameiosis). In spite of the reproductive complexity ofA. nidulans, parameiosis has only now been observed in this fungus. Since parameiosis was characterized by the occurrence of genetic recombination inside heterokaryotic hyphae, master strains (uvs +) anduvs mutants with high rate of both mitotic exchanges or chromosome nondisjunction were used to form heterokaryons. Two groups of mitotic segregants were recovered directly from heterokaryons — aneuploids and stable haploids. Heterokaryons formed withuvs mutants produced a higher number of parameiotic segregants compared to the heterokaryons formed withuvs + strains. Segregants were analyzed by nutritional markers, acriflavine resistance and conidial color. Normal meiotic behavior of haploid recombinants was observed.
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Baptista, F., Machado, M.F.P.S. & Castro-Prado, M.A.A. Alternative reproduction pathway inAspergillus nidulans . Folia Microbiol 48, 597–604 (2003). https://doi.org/10.1007/BF02993465
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DOI: https://doi.org/10.1007/BF02993465