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Studies on zinc- and cadmium-bound proteins in bovine kidneys by biochemical and neutron activation techniques

Biological Trace Element Research volume 26, pages 503–512 (1990)Cite this article

Abstract

Bovine kidneys were found to contain about 78 ppm Zn and 0.78 ppm Cd. Approximately 45% of Zn and 60% of Cd were present in the cytosol fraction. More than 95% of these two metals were bound to macromolecules. Both Zn- and Cd-protein complexes were observed to be stable between pH 7 and 10.5. Separation and characterization of these proteins were carried out using several chromatographic and electrophoretic techniques in conjunction with instrumental neutron activation analysis (INAA). The results showed the presence of at least four Zn-binding proteins with mol wt>300,000, 260,000, 89,000, and 27,000 and at least three Cd-binding proteins of mol wt>300,000, 32,000, and 13,000.

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Affiliations

  1. Trace Analysis Research Centre, Department of Chemistry, Dalhousie University, B3H 4J1, Halifax, NS, Canada

    C. K. Jayawickreme & A. Chatt

Authors
  1. C. K. Jayawickreme
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  2. A. Chatt
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Jayawickreme, C.K., Chatt, A. Studies on zinc- and cadmium-bound proteins in bovine kidneys by biochemical and neutron activation techniques. Biol Trace Elem Res 26, 503–512 (1990). https://doi.org/10.1007/BF02992706

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  • DOI: https://doi.org/10.1007/BF02992706

Index Entries

  • Zinc
  • cadmium
  • proteins
  • neutron activation
  • chromatography
  • electrophoretic
  • pH dependence
  • bovine kidney