Skip to main content
SpringerLink
Log in

Preparation of immobilized tannins for protein adsorption

  • Published:
Journal of Solid Phase Biochemistry Aims and scope Submit manuscript

Abstract

Preparation and adsorption specificity of tannins immobilized by covalent binding on water-insoluble matrices were investigated. Immobilized tannins were prepared by condensing cyanogen bromide activated tannins with aminohexyl derivatives of several kinds of matrices. The most suitable matrix for the immobilization of tannin was alkali-treated cellulose powder. The concentration of sodium hydroxide solution for alkali treatment influenced the adsorption capacity of immobilized tannin for a protein, but temperature and time for alkali treatment did not. Immobilized tannins having spacers of long chain length exhibited high adsorption capacity for a protein. Chinese gallotannin was the most favorable ligand for protein adsorption. The immobilization of tannin on aminohexyl matrices was also possible by using epichlorohydrin instead of cyanogen bromide. The maximum adsorption capacity of the immobilized tannin for a protein was about 50 mg/ml of the absorbent. Immobilized tannin adsorbed proteins specifically but did not absorb low molecular weight compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Grant, R. A. (1974) Process Biochemistry 9: 11–14.

    CAS  Google Scholar 

  2. Messing, R. A. (1974) Brewers Digest 46: 60–63.

    Google Scholar 

  3. Atkinson, A. (1973) Process Biochemistry 8: 9–12.

    CAS  Google Scholar 

  4. Baum, G., andLynn, M. (1973) Process Biochemistry 10: 14–17.

    Google Scholar 

  5. Tosa, T., Sano, R., Yamamoto, K., Nakamura, M., andChibata I. (1972) Biochemistry 11: 217–222.

    Article  CAS  Google Scholar 

  6. Cuatrecasas, P. (1970) J. Biol. Chem. (1970) 245: 3059–3065

    CAS  Google Scholar 

  7. Porath, J., andFornstedt N. (1970) J. Chromatogr. 51: 479–489.

    Article  CAS  Google Scholar 

  8. Inman, J. K., andDintzis, H. M. (1969) Biochemistry 8: 4074–4082.

    Article  CAS  Google Scholar 

  9. Hoare, D. G., andKoshland, D. E. (1966) J. Amer. Chem. Soc. 88: 2057–2058.

    Article  CAS  Google Scholar 

  10. Hoare, D. G., andKoshland, D. E. (1967) J. Biol. Chem. 242: 2447–2453.

    CAS  Google Scholar 

  11. Masaharu, K. (1949) Yakugaka Kenkyu 21: 129–132.

    Google Scholar 

  12. Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. (1951) J. Biol. Chem. 193: 265–275.

    CAS  Google Scholar 

  13. Cadavid, N. G., andPaladini, A. C. (1964) Anal. Biochem. 9: 170–174.

    Article  CAS  Google Scholar 

  14. Somogi, M. (1945) J. Biol. Chem. 160: 61–68.

    Google Scholar 

  15. Bock, R. M., andAlberty, R. A. (1953) J. Amer. Chem. Soc. 75: 1921–1925.

    Article  CAS  Google Scholar 

  16. Goodman, A. E., andStark, J. B. (1957) Anal. Chem. 29: 283–287.

    Article  Google Scholar 

  17. Kennedy, J. F. (1974) Adv. Carbohydr. Chem. Biochem. 29: 305–308.

    Article  CAS  Google Scholar 

  18. Wilchek, M., andMiron, T. (1976) Biochem. Biophys. Res. Commun. 72: 108–113.

    Article  CAS  Google Scholar 

  19. Watanabe, T.,Fujimura, M.,Mori, T.,Tosa, T., andChibata, I. (in press) J. Appl. Biochem.

  20. Watanabe, T., Mori, T., Tosa, T., andChibata, I. (1979) Biotech. Bioeng. 21: 477–486.

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Yuhsi Matuo

    Present address: Institute for Protein Research Osaka University, Yamada-kami, Suita, Osaka, Japan

Authors and Affiliations

  1. Department of Biochemistry, Research Laboratory of Applied Biochemistry Tanabe Seiyaku Co., Ltd., 16-89, Kashima-3-chome, Yodogawa-ku, Osaka, Japan

    Taizo Watanabe, Yuhsi Matuo, Takao Mori, Ryujiro Sano, Tetsuya Tosa & Ichiro Chibata

Authors
  1. Taizo Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yuhsi Matuo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takao Mori
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ryujiro Sano
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tetsuya Tosa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ichiro Chibata
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Watanabe, T., Matuo, Y., Mori, T. et al. Preparation of immobilized tannins for protein adsorption. Journal of Solid Phase Biochemistry 3, 161–174 (1978). https://doi.org/10.1007/BF02991844

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02991844

Keywords

Search

Navigation