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Radioimmunoassay of metallothionein in rabbit, rat, mouse, Chinese hamster, and human cells

Biological Trace Element Research volume 30, pages 245–256 (1991)Cite this article

Abstract

We describe a competitive, solid-phase radioimmunoassay for metallothionein, which employs a rabbit antiserum directed against rat MT-2 to detect metallothionein (MT) from several different species (rabbit, mouse, rat, Chinese hamster, and human). The lower limit of detection of the assay for rat MT-2 was 0.7 ng; for rabbit MT-2 it was 2 ng. The method is capable of measuring both isoforms of MT (MT-1 and MT-2). When MT levels in rat and mouse tissues were estimated with this RIA and the silver-saturation method, both assays gave the same pattern of MT induction in control and cadmium-treated animals. Both methods measured high levels of MT in human liver samples. Chinese hamster ovary cells induced with cadmium also showed elevated MT expression. The detectability of MTs from a broad range of species is facilitated by the use of solid-phase MT, which has an avidity for the antiserum similar to that of the MT in the tested sample.

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Affiliations

  1. Department of Pathology, Health Sciences Centre, University of Western Ontario, N6A 5C1, London, Ontario, Canada

    Martha E. I. Leibbrandt, James Koropatnick & M. George Cherian

  2. Department of Oncology, University of Western Ontario, 790 Commissioners Rd. East, N6A 4L6, London, Ontario, Canada

    James Koropatnick & John F. Harris

  3. Department of Microbiology and Immunology, University of Western Ontario, 790 Commissioners Rd. East, N6A 4L6, London, Ontario, Canada

    James Koropatnick & John F. Harris

Authors
  1. Martha E. I. Leibbrandt
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  2. James Koropatnick
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  3. John F. Harris
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  4. M. George Cherian
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Leibbrandt, M.E.I., Koropatnick, J., Harris, J.F. et al. Radioimmunoassay of metallothionein in rabbit, rat, mouse, Chinese hamster, and human cells. Biol Trace Elem Res 30, 245–256 (1991). https://doi.org/10.1007/BF02991419

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  • DOI: https://doi.org/10.1007/BF02991419

Index Entries

  • Radioimmunoassay
  • metallothionein
  • human
  • mouse
  • rat
  • silver-saturation method
  • liver
  • kidney