Journal of Solid-Phase Biochemistry

, Volume 3, Issue 1, pp 49–56 | Cite as

Study on the properties of dextran-linked adenine nucleotide derivatives

  • Chi-yu Lee


A simple method is described for the preparation of dextran-linked coenzyme derivatives. Several different 8-(6-aminohexyl)amino-adenine nucleotide coenzymes and their derivatives were covalently attached to dextran by incubation with bromohydroxypropyl derivatives of dextran at room temperature in an alkaline medium. The polymer-linked adenine nucleotide derivatives were separated from the free coenzyme derivatives by a Sephadex G-50 column. The prepared dextran derivatives have ligand densities ranging from 20 to 100 μmol/g of dextran derivatives depending on the conditions of coupling and derivatives. NMR studies revealed that proton resonances of the polymer-linked coenzymes exhibit short transverse relaxation times (T2) but long longitudinal relaxation times (T1) This phenomenon was interpreted in terms of the anisotropic motions of the dextran-bound coenzyme derivatives in which the fast axial motions and slow restricted transverse motions of the bound coenzyme derivatives are postulated. These observations could properly explain why the polymer-linked coenzymes exhibit lower biological activity, but similar binding affinity to most enzymes.


Dextran Proton Resonance Longitudinal Relaxation Time Ligand Density Anisotropic Motion 
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Copyright information

© Humana Press Inc. 1978

Authors and Affiliations

  • Chi-yu Lee
    • 1
  1. 1.Laboratory of Environmental Mutagenesis National Institute of Environmental Health Sciences Research Triangle ParkNorth Carolina

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