Abstract
Simultaneous application of high-molecular-weight active esters and polyvinylN,N- diethylbenzylamine was used as a basis for a continuous peptide synthesis via the polymeric reagents approach. Using the synthetic procedure developed, the hexapeptide Boc-L-Pro-L-Val-L-Lys[Z(p-NO2)]-L-Val-L-Tyr(Dnp)-L-Pro-OBzr1 and the tetrapeptide Boc-l-Lys[Z(p-NO2)]-L-Lys[Z(p-NO2)]-L-Arg(NO2)-L-Arg(NO2)-OBzr, corresponding to residues 19–24 and 15–18 of human ACTH, were synthesized in 63 and 70% overall yields, respectively.
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Stern, M., Kalir, R., Patchornik, A. et al. Toward mechanized peptide synthesis via polymeric reagents approach. Journal of Solid-Phase Biochemistry 2, 131–139 (1977). https://doi.org/10.1007/BF02991403
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DOI: https://doi.org/10.1007/BF02991403