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Covalent coupling of unsaturated compounds to thiol agrarose using γ-Radiation

A new method for preparation of adsorbents for affinity chromatography

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Abstract

Unsaturated compounds can be attached to thiol polymers by means of ΰ-radiation. By this method several agarose conjugates have been synthesized from thiol agarose gel and different ligand derivatives (testosterone, estradiol, 3-octenoic acid, and hexa-2,4-dienoic acid). The amount of fixed ligand is about 60-120 μmol/g of dried conjugate. The method described is a valuable complement to the currently available procedures for the preparation of adsorbents for affinity chromatography.

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Authors and Affiliations

  1. Institute of Biochemistry, University of Uppsala, Box 576, S-751 23, Uppsala, Sweden

    Johnny Brandt, Anders Svenson, Jan Carlsson & Håkan Drevin

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  1. Johnny Brandt
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  2. Anders Svenson
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  3. Jan Carlsson
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  4. Håkan Drevin
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Brandt, J., Svenson, A., Carlsson, J. et al. Covalent coupling of unsaturated compounds to thiol agrarose using γ-Radiation. Journal of Solid-Phase Biochemistry 2, 105–109 (1977). https://doi.org/10.1007/BF02991400

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  • DOI: https://doi.org/10.1007/BF02991400

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