Journal of Soils and Sediments

, Volume 2, Issue 4, pp 203–210 | Cite as

The interactive effects of chelator, fertilizer, and rhizobacteria for enhancing phytoremediation of heavy metal contaminated soil

  • Hong Chen
  • Teresa J. Cutright
Research Article


The role of chelator, fertilizer, and enriched rhizobacteria in facilitating Cd, Cr, and Ni accumulation byHelianthus annuus was studied. It was found that by adding a synthetic chelator, EDTA, the shoot concentrations of Cd and Ni were significantly increased from 34.2 mg kg-1 and 14.5 mg kg-1 to 115 mg kg-1 and 117 mg kg-1, respectively. However, the total biomass of plants was drastically decreased by 50 to 60%. Compared with this treatment, inoculating enriched rhizobacteria to plants grown under similar conditions maintained the surged shoot concentrations of Cd and Ni while increasing the plants biomass by more than 1.6-fold. It was also found that introducing a commercial fertilizer, Hydro-Gro®, to plants significantly-increased the Ni accumulation by 3-fold and the plant biomass by 1.43-fold. These results suggest that combing fertilizers, chelators and/or rhizobacteria might provide a more effective approach for enhancing phytoremediation.


Chelator fertilizer heavy metals phytoremediation enhancement rhizobacteria 


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  1. [1]
    Raskin I, Smith RD, Salt DE (1997): Phytoremediation of Metals: Using Plants to Remove Pollutants from the Environment. Current Opinion in Biotechnology 8, 221–226CrossRefGoogle Scholar
  2. [2]
    Salt DE, Blaylock M (1995): Phytoremediation: A Novel Strategy for the Removal of Toxic Metals from the Environment using Plants. Biotechnology 13, 468–474CrossRefGoogle Scholar
  3. [3]
    Alloway BJ (ed) (1995): Heavy Metals in Soils (2nd Edition) Chapman & Hall, LondonGoogle Scholar
  4. [4]
    Merian E (ed) (1991): Metals and Their Compounds in the Environment. VCH Publishers, Inc., New YorkGoogle Scholar
  5. [5]
    US Dept of Energy (1994): Summary Report of a Workshop in Phytoremediation Research Needs. DOE/EM-0224Google Scholar
  6. [6]
    Khan AG, Kuek C, Chaudhry TM, Khoo CS, Hayes WJ (2000): Role of Plants, Mycorrhizae and Phytochelators in Heavy Metal Contaminated Land Remediation. Chemosphere 41 197–207CrossRefGoogle Scholar
  7. [7]
    Blaylock MJ (1997): Enhanced Accumulation of Pb in Indian Mustard by Soil-Applied Chelating Agents, Environmental Science & Technology 31, 860–865Google Scholar
  8. [8]
    Salt DE, Smith RD, Raskin I(1998): Phytoremediation. Annual Review of Plant Physiology and Plant Molecular Biology 49, 643–668CrossRefGoogle Scholar
  9. [9]
    Chen H(2000): Phytoremediation of Soil Contaminated with Cd, Cr, and Ni. M.S. Thesis, University of AkronGoogle Scholar
  10. [101.
    Robinson BH (1998): The Potential ofThlaspi caerulescens for Phytoremediation of Contaminated Soils. Plant and Soil 203 47–56CrossRefGoogle Scholar
  11. [11]
    Huang JW, Chen JJ, Berti WB, Cuningham SD (1997): Phytoremediation of Lead-Contaminated Soils: Role of Synthetic Chelates in Lead Phytoextraction. Environmental Science and Technology 31, 800–805CrossRefGoogle Scholar
  12. [12]
    Robinson BH, Brooks RR, Clothier BE (1999): Soil Amendments Affecting Nickel and Cobalt Uptake byBerkheya coddii: Potential Use for Phytomining and Phytoremediation. Annals of Botany 84, 6689–6994CrossRefGoogle Scholar
  13. [13]
    Robinson BH, Brooks RR, Howes AW, Kirkman JH, Gregg P (1997): The Potential of the High-Biomass Nickel HyperaccumulatorBerkeya coddii for Phytoremediation and Phytomining. Journal Geochemical Exploration 60, 115–126CrossRefGoogle Scholar
  14. [14]
    Ebbs SD, Lasat MM, Brady DJ, Cornish J, Gordon R, Kochian LV (1997): Heavy Metals in the Environment: Phytoextraction of Cadmium and Zinc from a Contaminated Soil. Journal Environmental Quality 26, 1424–1430Google Scholar
  15. [15]
    Bennett FA, Tyler EK, Brooks RR, Gregg P, Stewart RB (1998): Fertilization of Hyperaccumulators to Enhance their Potential for Phytoremediation and Phytomining. In Plants that Hyperaccumulate Heavy Metals. CAB International, WallingfordGoogle Scholar
  16. [16]
    Crooke WM, Inkson RHE(1995): The Relationship Between Nickel Toxicity and Major Nutrient Supply. Plant and Soil 6, 1–15CrossRefGoogle Scholar
  17. [17]
    Gabberielli R, Pandolfini T (1984): Effect of Mg2+ and Ca2+ on the Response to Nickel Toxicity in a Serpentine endemic and Nickel-accumulating Species. Physiologia Plantarum 62, 540–544CrossRefGoogle Scholar
  18. [18]
    Kozyrovska N (1996): Novel Inoculants for an Environmentally-friendly Crop Production. Resources, Conservation and Recycling 18, 79–85CrossRefGoogle Scholar
  19. [19]
    Salt DE, Benhamou N, Leszczyniecka M, Raskin I, Chel I (1999): A Possible Role for Rhizobacteria in Water Treatment by Plant Roots. International J Phytoremediation 1, 67–79CrossRefGoogle Scholar
  20. [20]
    De Souza MP, Chu D, Zhao M, Zayed AM, Ruzin SE, Schichnes D, Terry N (1999): Rhizosphere Bacteria Enhance Selenium Accumulation and Volatilization by Indian Mustard. Plant Physiology 119, 565–573CrossRefGoogle Scholar
  21. [21]
    Van Der Lelie D (2000): The role of Bacteria in the Phytoremediation of Heavy Metals. In Phytorem. Contam. Soil Water. Lewis Publishers, Boca Raton, FL, pp 265–281Google Scholar
  22. [22]
    Tan KH (1995): Soil Sampling, Preparation, and Analysis. Marcel Dekker, Inc., New YorkGoogle Scholar
  23. [23]
    Page AL (ed) (1982): Methods of Soil Analysis (Part 2): Chemical and Microbiological Properties. American Society of Agronomy, IncGoogle Scholar
  24. [24]
    Carter MR (ed) (1993): SoilSampling and Methods of Analysis, Lewis PublishersGoogle Scholar
  25. [25]
    Mench MJ (1994): A Mimicked In-Situ Remediation Study of Metal-Contaminated Soils with Emphasis on Cadmium and Lead. J Environmental Quality 23, 58–63CrossRefGoogle Scholar
  26. [26]
    Stotzky G, Bollag JM (l996): Soil Biochemistry, Vol 9, Marcel Dekker, New YorkGoogle Scholar
  27. [27]
    Goodhue CT (1982): The Methodology of Microbial Transformation of Organic Compounds. Microbial Transformation Bioact Compound 1,9–444Google Scholar
  28. [28]
    Siciliano SD, Germida JJ (1999): Enhanced Phytoremediation of Chlorobenzoates in Rhizosphere Soil. Soil Biology and Biochemistry 31, 299–305CrossRefGoogle Scholar
  29. [29]
    Zheljazkov VD, Erickson NE (1996): Studies on the Effect of Heavy Metals (Cd, Pb, Cu, Mn, Zn, and Fe) Upon the Growth, Productivity, and Quality of Lavender Production. J Essential Oil Res. 8, 259–274Google Scholar
  30. [30]
    Pahlsson A (1989): Toxicity of Heavy Metals (Zn, Cu, Cd, Pb) To Vascular Plants: A Literature Review. Water, Air, and Soil Pollution 47, 287–319CrossRefGoogle Scholar
  31. [31]
    Salt DE, Prince R, Pickering IJ, Raskin I (1995): Mechanisms of Cadmium Mobility and Accumulation in Indian Mustard. Plant Physiology 109, 1427–1433Google Scholar

Copyright information

© Ecomed Publishers 2002

Authors and Affiliations

  1. 1.Department of Civil EngineeringUniversity of AkronAkronUSA

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