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Comparison of high performance liquid chromatography and fluorometric ocean colour pigments

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Abstract

The accuracy of satellite derived chlorophylla (chla) using empirical algorithms (OC2 and OC4) is about ± 30–35%, which is attributed mainly to the sensor and atmospheric constraints and also the bio-optical algorithms. However errors inin situ measurement of chla may also contribute to the retrieval accuracy. The fluorometric method of chla measurement can significantly under or overestimate chla concentrations. This is mainly because of the overlap of the absorption and fluorescence bands of co-occurring chlorophyllsb andc, chlorophyll degradation products, and accessory pigment. Accurate chla measurements are important for validating satellite derived chla accuracy and algorithm development. The focus of this study was to understand the discrepancy between fluorometric and HPLC (High Performance Liquid Chromatography) derived chla using unialgal cultures, natural field samples from Bedford Basin and samples from MinOx cruise to analyse divinyl chla. Approximately 50% underestimation of chla both in the natural samples as well as cultured samples has been observed by fiuorometer. The results of MinOx cruise data indicated shifting of the blue absorption maxima towards longer wavelengths (~450nm), which is consistent with high concentration of divinyl chla (chla 2) associated with prochlorophytes.

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  1. Marine and Water Resources Group, Space Applications Centre, Indian Space Research Organisation, 380015, Ahmedabad, India

    Beena Kumari

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  1. Beena Kumari
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Correspondence to Beena Kumari.

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Kumari, B. Comparison of high performance liquid chromatography and fluorometric ocean colour pigments. J Indian Soc Remote Sens 33, 541–546 (2005). https://doi.org/10.1007/BF02990739

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  • DOI: https://doi.org/10.1007/BF02990739

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