Abstract
Earlier studies have shown a lower degree of lodgement and early survival of tumor cells in muscle than in liver after infusion via the femoral artery and portal vein, respectively. A possible explanation to this difference might be that the tumor cells are mechanically destroyed, and thus die more rapidly in muscle because they enter this capillary network at a much higher flow velocity. In the present study, the effect on early tumor cell (rat fibrosarcoma) survival of a high and a low flow velocity/deformation rate was evaluated in micropore (5 μm) filters, using isotope (Cr51) technique. These experiments were combined with scanning electron microscopic (SEM) analyses. The filter experiments showed no significant differences in the rate of cell death in the filters between tumor cells subjected to high or low deformation rates, and there were no qualitative differences in tumor cell appearance in the SEM study. It is, therefore, concluded that the difference in tumor cell lodgement and survival between muscle and liver is not primarily caused by differences in the rate of cell deformation upon entry of the organ capillary network.
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References
Fidler, I. J. (1970),J. Nat. Cancer Inst. 45, 773–782.
Weiss, L. and Dimitrov, D. S. (1984),Cell Biophysics 6, 9–22.
Bagge, U., Skolnik, G., and Ericson, L. E. (1983),J. Cancer Res. Clin. Oncol. 105, 134–140.
Gabor, H. and Weiss, L. (1985),Invasion Metastasis 5, 71–83.
Folkow, B. and Neil, E. (1971),Circulation, Oxford University Press.
Bagge, U. (1975),White Blood Cell Rheology, Dissertation, University of Göteborg, Göteborg, Sweden.
Blomqvist, G., Skolnik, G., Braide, M., Bjursten, L. M., Blixt, Å, and Bagge, U. (1988),Clin. Expl. Metastasis 6, 285–289.
Weiss, L. and Schmid-Schönbein, G. W. (1989),Cell Biophysics 14, 187–215.
Ivarsson, L. and Rudenstam, C. M. (1975),Eur. Surg. Res. 8, 326–340.
Nannmark, U., Johansson, B. R., and Bagge, U. To be published.
Gaehtgens, P. (1977), Handbuch der allgemeinen Pathologie III/7; Mikrozirkulation/Microcirculation. Ed. H. Meessen. Springer-Verlag, Berlin.
Inoué, T. and Osatake, H. (1988),Arch. Histol. Cytol. 51, 53–59.
Weiss, L. (1989),Clin. Expl. Metastasis 7, 483–491.
Bagge, U., Johansson, B. R., and Olofsson, J. (1977),Adv. Microcirc. 7, 18–28.
Schmid-Schönbein, G. W., Shih, Y. Y., and Chien, S. (1980),BLOOD 56, 866–857.
Weiss, L., Harlos, J. P., and Elkin, G. (1989),Int. J. Cancer 44, 143–148.
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Nannmark, U., Blomqvist, G., Braide, M. et al. The influence of different flow velocities on tumor cell survival in micropore filters. Cell Biophysics 17, 153–161 (1990). https://doi.org/10.1007/BF02990494
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DOI: https://doi.org/10.1007/BF02990494