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The surface free energy ofLeishmania mexicana amazonensis

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Abstract

Surface charge ofLeishmania mexicana amazonensis was investigated by direct zeta-potential determination and ultrastructural cytochemistry, and its surface tension was studied by measurements of the advancing contact angle formed by the parasite monolayers with drops of liquids of different polarities. Both virulent and avirulent promastigotes exhibited negatively charged surfaces with a zeta-potential of about — 15 mV. Treatment of these cells with trypsin, alkaline phosphatase, or phospholipase C rendered their surfaces less negatively charged, whereas neuraminidase did not alter the parasite negativeness. Cytochemically, we could observe a reduction in the cationized ferritin binding after the parasite treatment with each of the former enzymes, but not with neuraminidase. The surface free energy of parasites was calculated by taken to account the London dispersion, the Keeson dipole-dipole, and the Debye dipole-induced forces, as well as the surface polarity of the parasites and their zeta-potentials, by considering their adhesion to polystyrene surfaces. The ΔG values of −6.4 and −18.1 mJ m−2 were obtained for avirulent and virulent promstigotes, respectively.

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Authors and Affiliations

  1. Laboratório de Ultraestrlutura Celular e Microscopia Eletrônica, Departamento de Biofísica Celular e Parasitologia, Instituto de Biofísica Carlos Chagas Filho, Brazil

    Fernando Costa e Silva Fllho, Elvira Maria Breier Saraiva, Marcos André vannier Santos & Wanderley de Souza

  2. Departamento de Imunologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, CCS - Bloco G, Ilha do Fundāo, 21941, Rio de Janeiro, Brazil

    Elvira Maria Breier Saraiva

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  1. Fernando Costa e Silva Fllho
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  2. Elvira Maria Breier Saraiva
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  3. Marcos André vannier Santos
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  4. Wanderley de Souza
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Fllho, F.C.e.S., Saraiva, E.M.B., Santos, M.A.v. et al. The surface free energy ofLeishmania mexicana amazonensis . Cell Biophysics 17, 137–151 (1990). https://doi.org/10.1007/BF02990493

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  • DOI: https://doi.org/10.1007/BF02990493

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