Abstract
The interaction of the vanadyl (IV) cation with reduced glutathione (GSH) has been investigated by electronic absorption spectroscopy, at different metal-to-ligand ratios and pH values. The interaction depends strongly on the initial VO2+/GSH ratio. Starting with a tenfold GSH excess, coordination takes place through the two carboxylate groups of the ligand, generating (at pH=7) a blue 1∶2 VO2+/GSH complex; this stoichiometry could be confirmed by photometric titration experiments. Higher GSH concentrations produce a violet complex, which can also be obtained by addition of GSH to the blue species. Some measurements with the three component amino acids of GSH, as well as results obtained from the VO −3 /GSH system, allowed a wider insight into the characteristics of this violet complex, in which the cation interacts with S and N atoms of the peptide.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
A. L. Lehninger,Principles of Biochemistry, Worth, New York, 1982.
D. L. Rabenstein, R. Guevremont, and Ch. A. Evans,Metal Ions in Biological Systems (H. Sigel, ed.), vol. 9, Marcel Dekker, New York, 1979, p. 103.
B. Mannervick,Chem. Scripta 26B, 281 (1986).
H. Sies,Naturwissenschaften 76, 57 (1989).
K. Kustin and I. G. Macara,Comments Inorg. Chem. 2, (1982).
N. D. Chasteen,Struct. Bonding 53, 105 (1983).
I. G. Macara, K. Kustin, and L. C. Cantley, Jr.,Biochim. Biophys. Acta 629, 95 (1980).
M. Degani, M. Gockin, S. J. D. Karlish and Y. Schechter,Biochemistry 20, 5795 (1981).
C. J. Ballhausen and H. B. Gray,Inorg. Chem. 1, 111 (1962).
A. B. P. Lever,Inorganic Electronic Spectroscopy, 2nd Ed., Elsevier, Amsterdam, 1984.
K. Wühtrich,Helv. Chim. Acta 48, 779 (1965).
E. J. Baran, A. H. Jubert, and A. L. Rocha,J. Raman Spectr. 20, 801 (1989).
K. A. Connors,Binding Constants, Wiley, New York, 1987.
M. Delfini, E. Gaggelli, A. Lepri, and G. Valensin,Inorg. Chim. Acta 107, 87 (1985).
H. Tomiyasu and G. Gordon,J. Coord. Chem. 3, 47 (1973).
C. R. Johnson and R. E. Shepherd,Bioinorg. Chem. 8, 115 (1979).
H. Sakurai, S. Shimomura, and K. Ishizu,Inorg. Chim. Acta 55, L 67 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ferrer, E.G., Williams, P.A.M. & Baran, E.J. A spectrophotometric study of the VO2+-glutathione interactions. Biol Trace Elem Res 30, 175–183 (1991). https://doi.org/10.1007/BF02990352
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02990352