Summary
In order to clarify how PGE2 regulates gastric mucosal integrity, we examined the effects of PGE2 on ethanol-caused injury of isolated gastric chief cells, cultured gastric mucous cells from guinea pigs and Balb/c 3T3 fibroblasts. Pretreatment of these cells with PGE2 reduced ethanol-caused injury of the cells. Furthermore, pretreatment of gastric mucous cells with indomethacin enhanced ethanol-caused injury, suggesting that endogenous PGE2 may be involved in the cell protection. PGE2 stimulated an increase in diacylglycerol (DG) accumulation in chief cells and treatment of chief cells with synthetic DG reduced the injury of the cells. However, DG accumulation was not observed in gastric mucous cells treated with PGE2. Therefore PGE2 may protect the cells from injury through a variety of mechanisms. In addition, PGE2 enhanced the survival of the quiescent fibroblasts cultured in the absence of serum, while PGE2 had no survival enhancing effect on gastric mucous cells. These results sugest that the mechanism by which PGE2 preserves the cell viability may depend on not only cell types used but also how the cells are injured.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Cheung LY. Topical affects of 16.16 dimethyl prostaglandin E2 on gastric blood flow in dogs. Am J Physiol 1980;238: G514-G519.
Miller TA. Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms. Am J Physiol 1983;245: G601-G623.
Lichtenberger LM, Graziane LA, Dial EJ, et al. Role of surface active phospholipids in gastric cytoprotection. Science 1983;219: 1327.
Robert A, Nezamis JE, Lancaster C, et al. Protection by prosta-glandins in rat. Prevention of gastric necrosis produced by alcohol, HCL, NaOH, hypertonic NaCl, and thermal injury. Gastroenterology 1979;77: 433–443.
Tarnawski A, Hollander D, Stachura J, et al. Prostaglandin protection of the human gastric mucosa against alcohol-induced injury. Scand J Gastroenterol 1986;21: 165–169.
Koyama S, Rennard SI, Classen L, et al. Dibutyryl cAMP, prosta-glandin E2, and antioxidants protect cultured bovine bronchial epithelial cells from endotoxin. Am J Physiol 1991;261: L126-L132
Colletti LM, Burton GD, Campbell Jr. DA. Prostaglandin E2 protects the isolated perfused Rabbit liver from an oxygen free radical-induced injury. Transplant Proc 1990;22: 2381–2383.
Gerschenson LE, Rotello RJ. Apoptosis: a different type of cell death. FASEB J 1992;6: 2450–2455.
Sakamoto C, Matozaki T, Nagao M, et al. Regulation of free cyto-solic Ca2+ in the isolated guinea pig gastric chief cells. Biochem Biophys Res Commun 1987;142: 865–871.
Tamm I, Kikuchi T. Insulin-like growth factor I (IGF-1), insulin, and epidermal growth factor (EGF) are survival factor for density-inhibited quiescent Balb/c-3T3 murine fibroblasts. J Cell Physiol 1990;143: 494–500.
Farese RV, Konda TS, Davis JS, et al. Insulin rapidly increases diacylglycerol by activating de novo phosphatidic acid synthesis. Science 1987;236: 586.
Terano A, Ota S, Mach T, et al. Prostaglandin protects against taurocholate-induced damage to rat gastric mucosal cell culture. Gastroenterology 1986;92: 669–677.
Mutoh H, Hiraishi H, Ota S, et al. Role of oxygen radicals in ethanol-induced damage to cultured gastric mucosal cells. Am J Physiol 1990;258: G603-G609
Miller TA, Li D, Kuo Y, et al. Nonprotein sulfhydryl compounds in canine gastric mucosa: effects of PGE2 and ethanol. Am J Physiol 1985;249: G137-G144.
Szabo S, Trier JS, Frankel PW. Sulfhydryl compounds may mediate gastric cytoprotection. Science 1981;214: 200–202.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sakamoto, C., Matsuda, K., Konda, Y. et al. PGE2 protects isolated cells against injury through multiple mechanisms. Gastroenterol Jpn 28 (Suppl 5), 122–126 (1993). https://doi.org/10.1007/BF02989222
Issue Date:
DOI: https://doi.org/10.1007/BF02989222