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Tracking of phenol degrading genotype

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A simple and rapid protocol has been developed for monitoring of the phenol degrading population in the environmental samples. PCR protocol uses the total DNA prepared from the samples as a template in the amplification reaction. Primers have been designed from the sequence data for Dmp and Phe operon encoding multi-component and single component phenol oxidizing system, respectively. The phenol degrading genotype in various samples have been demonstrated using the developed PCR protocol.

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Correspondence to Hemant J. Purohit.

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Kapley, A., Purohit, H.J. Tracking of phenol degrading genotype. Environ Sci & Pollut Res 8, 89–90 (2001).

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