Abstract
Trichlorofluoromethane, in concentrations of 80, 400, 2 000, 10 000 and 50 000 ppm, was administered to rats of both sexes (Sprague Dawley) by inhalation exposure.
2 000 ppm in air (= 11 200 mg/m3) amount to twice the MAK value of 1 000 ppm. At exposure times of 4 hours this corresponds to the MAK value defined for an 8 hour workday.
Unscheduled DNA synthesis (UDS) was measured in single-cell suspensions of hepatocytes, pulmonary epithelial cells and lymphocytes of the spleen, respectively.
In the pulmonary cells concentrations of 2 000, 10 000 and 50 000 ppm of freon 11 lead to a significantly increased mean silver grain count compared to a negative control group.
In spleen and liver cells increasing concentrations of R 11 tend to increase the incorporation of thymidine into the DNA of the cells. These changes of the extent of unscheduled DNA synthesis can, however, not be statistically verified.
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Winker, N., Klein, W., Weniger, P. et al. UDS-test with freon 11 (R-11). Environ. Sci. & Pollut. Res. 2, 233–236 (1995). https://doi.org/10.1007/BF02986772
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DOI: https://doi.org/10.1007/BF02986772