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Inhibition of ethoxyresorufin-O-deethylase (EROD) activity in mixtures of 2,3,7,8-tetrachlorodibenzo-p-dioxin and polychlorinated biphenyls

EROD acitivity as biomarker in TCDD and PCB risk assessment

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Abstract

Induction of ethoxyresorufin-O-deethylase (EROD) activity and porphyrin accumulation shows different structure-activity relationships for different polychlorinated biphenyls (PCBs) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Interactions between the two responses can strongly influence the induction and activity of EROD. The results support the conclusion that there are nonadditive interactions between nondioxin-like PCBs and dioxin-like compounds. The interaction between EROD activity and the porphyrin biosynthesis makes the prerequisite of additivity in the toxic equivalency factor concept for environmental mixtures highly spurious. Inhibition of EROD activity caused by non-dioxin like compounds could have a significant impact on the value of EROD activity as a biomarker in the present methods of risk assessment for these compounds.

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Authors and Affiliations

  1. Institute of Environmental Chemistry, Umeå University, S-901 87, Umeå, Sweden

    Mats Tysklind, Patrik Andersson & Christoffer Rappe

  2. Research Institute of Toxicology, University of Utrecht, P.O. Box 80.176, NL-3508 TD, Utrecht, The Netherlands

    Albertus Bart T. C. Bosveld, Eeske Verhallen, Theo Sinnige, Willem Seinen & Martin van den Berg

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  1. Mats Tysklind
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  2. Albertus Bart T. C. Bosveld
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  3. Patrik Andersson
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  4. Eeske Verhallen
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  5. Theo Sinnige
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  6. Willem Seinen
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  7. Christoffer Rappe
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  8. Martin van den Berg
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Tysklind, M., Bosveld, A.B.T.C., Andersson, P. et al. Inhibition of ethoxyresorufin-O-deethylase (EROD) activity in mixtures of 2,3,7,8-tetrachlorodibenzo-p-dioxin and polychlorinated biphenyls. Environ. Sci. & Pollut. Res. 2, 211–216 (1995). https://doi.org/10.1007/BF02986768

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  • DOI: https://doi.org/10.1007/BF02986768

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