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Clonal Analysis of Granulocyte-Monocyte Colony-Forming Unit Cells with the Human Androgen Receptor Gene in Chronic Myeloid Leukemia

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Abstract

Coexistence of Philadelphia chromosome-negative (Ph-) progenitors with the Ph+ clone in the early chronic phase of chronic myeloid leukemia (CML) has been documented in previous reports. A different evaluation of methods is needed to justify the clonality of the residual Ph- progenitors. Therefore, the X chromosome inactivation patterns in individual granulocyte-monocyte colony-forming unit (CFU-GM) colonies were studied with the clonality assay for the human androgen receptor gene. A prerequisite for this evaluation was the validation of T-lymphocytes and buccal cells as control cells representing the constitutional lyonization. The percentages of polyclonal CFU-GM cells were determined in 9 Ph[su+] women with CML and in 5 healthy women. Results of the clonal analysis of CFU-GM colonies were compared with those from reverse transcriptase-polymerase chain reaction analysis of single colonies forBCR/ABL transcripts. Both methods of CFU-GM cell analysis were in agreement regarding the presence of variable proportions (0%–94%) of normal cells in CML. Our results suggest that (a) T-cells and buccal cells have potential for use as controls for the clonal analysis of CML cases and (b) this method can evaluate the frequency of polyclonal/clonal CFU-GM cells in CML cases and is applicable to the analysis of myeloid clonal disorders that lack specific molecular markers.

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Authors and Affiliations

  1. First Department of Medicine, University of Athens School of Medicine, Athens, Greece

    Salem Akel, Despina Mavroyanni, Xenophon Yataganas, Evagelos Terpos, John Meletis, Kostas Anargyrou, Niki Stavrogianni, Gerasimos-Alexander Pangalis, Dimitris Loukopoulos & Nora Viniou

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  1. Salem Akel
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  2. Despina Mavroyanni
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  3. Xenophon Yataganas
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  4. Evagelos Terpos
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  8. Gerasimos-Alexander Pangalis
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  10. Nora Viniou
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Correspondence to Nora Viniou.

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Akel, S., Mavroyanni, D., Yataganas, X. et al. Clonal Analysis of Granulocyte-Monocyte Colony-Forming Unit Cells with the Human Androgen Receptor Gene in Chronic Myeloid Leukemia. Int J Hematol 77, 476–481 (2003). https://doi.org/10.1007/BF02986616

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  • DOI: https://doi.org/10.1007/BF02986616

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