Abstract
The genomic DNA was prepared from trout liver which was treated with 3-methycholanthrene, and cloned into lambda EMBL3, atBamH1 site. The genomic library constructed via infections of these recombinant phages intoE. coli K802, and screened by the most 5′-portion of trout CYP1A1 cDNA. After the screening of 109 clones of the amplified library, 12 positive clones were isolated, and subjected to further screenings. The results of southern, blot hybridization of genomic DNA prepared from the positive clone showed the presence of a single gene ofCYP1A1, and 3.5 KbPstI fragment that hybridizes with the most 5′-region DNA of CYP1A1 cDNA. The restriction map ofPstI fragment was determined by the restriction digestion with various enzymes. The nucleotide sequence of the upstream genomic DNA ofCYP1A1 was determined by DNA sequencing of exonuclease III unidirectionally deletedPstI fragment DNA using [35]dATP. This paper presented the upstream genomic DNA of CYP1A1 contained a part of coding region which was about 351 base pairs (from ATG toPstI site at 3563).
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Roh, Y.N., Sheen, Y.Y. Isolation of 5′-untranslational region of troutCYP1A1 gene. Arch. Pharm. Res. 19, 450–455 (1996). https://doi.org/10.1007/BF02986010
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DOI: https://doi.org/10.1007/BF02986010