Abstract
Objective: To investigate the serum levels of soluble macrophage colony-stimulating factor receptor (M-CSFsR) in normal subjects and patients with hematological diseases and its clinical implications in hematological diseases. Methods: The concentration of M-CSFsR was determined by ELISA. The serum M-CSFsR was identified and characterized by immunoprecipitation and Western blotting. Results: The mean serum level of M-CSFsR of 123 normal individuals was 0.48 ng/ml ± 0.41 ng/ml. Immunoprecipitation and Western blotting assay revealed a ∼ 90kD band of serum M-CSFsR. The mean serum M-CSFsR level of 60 patients with acute lymphoblastic leukemia (ALL), 36 patients with acute myeloblastic leukemia (AML), 13 patients with myelodysplastic syndrome (MDS) and 42 patients with aplastic anemia (AA) .were 0.22 ng/ml±0.23 ng/ml, 0.17 ng/ml±0.16 ng/ml, 0.19 ng/ml±0.16 ng/ml and 0.23 ng/ml±0.21 ng/ml, respectively, which were significantly lower than that of normal subjects (P=0.002 ,P<0.0001,P<0.0001 andP<0.0001). The mean serum M-CSFsR level of 51 idiopathic thrombocytopenic purpura (ITP) patients was significantly higher than that of normal subjects (2.05 ng/ml±2.75 ng/ml,P<0.0001). Conclusion: The serum M-CSFsR levels of patients with ALL, AML, MDS and AA were significantly lower, while the level of patients with ITP was significantly higher than that of normal individuals. Patients with severe ITP (platelet count<30×l09/L) had the highest M-CSFsR level. It suggested that the abnormal levels of serum M-CSFsR may associate with some hematological diseases and may contribute to the pathological process.
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Foundation item: This work was supported by National “863” High Technology Program of China ( 102-11-01-03).
Biography: RAO Qing (1965-), assistant professor, Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, majors in biophysics.
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Rao, Q., Han, Js., Sha, Xj. et al. Determination of serum soluble macrophage colony-stimulating factor receptor levels in patients with hematological diseases. Chin J Cancer Res 13, 185–189 (2001). https://doi.org/10.1007/BF02983882
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DOI: https://doi.org/10.1007/BF02983882
Key words
- Macrophage colony-stimulating factor
- Receptor
- Enzyme linked immunosorbent assay
- Immunoprecipitation
- Western blotting
- Leukemia
- Idiopathic thrombocytopenic purpura