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International Journal of Hematology

, Volume 78, Issue 3, pp 226–232 | Cite as

Radiation Protective Effect of an Extract fromChelidonium majus

  • Jie-Young Song
  • Hyun-Ok Yang
  • Ji-Young Shim
  • Ji-Yeon Ahn
  • Young-Soo Han
  • In-Sung Jung
  • Yeon-Sook Yun
Progress in Hematology

Abstract

We earlier reported that CM-AIa isolated fromChelidonium majus had mitogenic activity, generated lymphokine-activated killer cells, and increased the number of granulocyte-macrophage colony-forming cells (GM-CFC). In an extended effort to search for other immunostimulatory effects, we evaluated the protective effects of in vivo injected CM-AIa against irradiation. CM-AIa was found to increase the number of bone marrow cells, spleen cells, GM-CFC, and platelets in irradiated mice. In addition, this agent induced endogenous production of cytokines such as interleukin 1 and tumor necrosis factor a, which are required for hematopoietic recovery. We also demonstrated that CM-AIa treatment 24 hours before irradiation protected mice with 80% survival at lethal dose 100/15. These findings indicate that CM-AIa may be a useful agent for reducing the time needed for reconstitution of hematopoietic cells after irradiation treatment.

Key words

Chelidonium majus Immunomodulator Hematopoietic Irradiation 

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References

  1. 1.
    Chirigos MA, Mastrangelo ML, Mitchell M, Krim M.Progress in Cancer Research and Therapy: Mediation of Cellular Immunity in Cancer by Immune Modifier. Vol 19. New York: Raven; 1982.Google Scholar
  2. 2.
    Hersh EM, Chirigos MA, Masrtangelo M J.Progress in Cancer Research and Therapy: Augmenting Agents in Cancer Therapy. Vol 16. New York: Raven; 1981.Google Scholar
  3. 3.
    Blackett NM, Marsh JC, Gordon MY, Okell SF, Aguado M. Simultaneous assay by six methods of the effect of haemopoietic precursor cells of adriamycin, methyl-CCNU,60Co γ-rays, vinblastine, and cytosine arabinoside.Exp Hematol. 1978;6:2–8.PubMedPubMedCentralGoogle Scholar
  4. 4.
    Elkind MM. Repair processes in radiation biology.Radiat Res. 1984;100:425–429.CrossRefGoogle Scholar
  5. 5.
    Behling UH. The radioprotective effect of bacterial endotoxin. In: NowotnyA, ed.Beneficial Effects of Endotoxin. New York: Plenum; 1983.Google Scholar
  6. 6.
    Song JY, Yang HO, Pyo SN, Jung IS, Yi SY, Yun YS. Immunomodulatory activity of protein-bound polysaccharide extracted fromChelidonium majus.Arch Pharm Res. 2002;25:158–164.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Till JE, McCulloch EA. Early repair processes in marrow cells irradiated and proliferating in vivo.Radiat Res. 1963;18:96–99.CrossRefPubMedGoogle Scholar
  8. 8.
    Talmadge JE, Herberman RB. The preclinical screening laboratory: evaluation of immunomodulatory and therapeutic properties of biological response modifiers.Cancer Treat Rep. 1986:70:171–182.PubMedPubMedCentralGoogle Scholar
  9. 9.
    Prasard KN.Handbook of Radiology. Boca Raton FL: CRC Press; 1984.Google Scholar
  10. 10.
    Ross WM, Peeke J. Radioprotection conferred by dextran sulfate given before irradiation in mice.Exp Hematol. 1986;14:147–155.PubMedGoogle Scholar
  11. 11.
    Ainsworth EJ. From endotoxins to newer immunomodulators: survival-promoting effects of microbial polysaccharide complexes in irradiated animals.Pharmacol Ther. 1988;39:223–241.CrossRefPubMedGoogle Scholar
  12. 12.
    Patchen ML, MacVittie TJ. Stimulated hemopoiesis and enhanced survival following glucan treatment in sublethally and lethally irradiated mice.Int J Immunopharmacol. 1985;7:923–932.CrossRefPubMedGoogle Scholar
  13. 13.
    Uglyanitsa KN, Nefydov LI, Doroshenko YM, et al. Ukrain: a novel antitumor drug.Drugs Exp Clin Res. 2000;16:341–456.Google Scholar
  14. 14.
    Santavy F, Alkaloids F. In: StahlE, ed.Thin-Layer Chromatography. Berlin: Springer; 1973:421–471.Google Scholar
  15. 15.
    Kalechman Y, Zuloff A, Albeck M, Strassmann G, Sredni B. Role of endogenous cytokines secretion in radioprotection conferred by the immunomodulator ammonium trichloro(dioxyethylene-O- O′)tellurate.Blood. 1995;85:1555–1561.PubMedGoogle Scholar
  16. 16.
    Nakamura W, Kankura T, Eto H. Occult blood appearance in feces and tissue hemorrhage in mice after whole body x-irradiation.Radiat Res. 1971;48:169–178.CrossRefPubMedGoogle Scholar
  17. 17.
    Nakamura W. Effect of thrombocytes-rich plasma on x-ray induced bone marrow death in mice.Radiat Res. 1973;55:118–128.CrossRefPubMedGoogle Scholar
  18. 18.
    Neta R, Douches SD, Oppenheim JJ. Interleukin 1 is a radioprotector.J Immunol. 1986;136:2483–2485.PubMedGoogle Scholar
  19. 19.
    Neta R, Oppenheim JJ, Douches SD. Interdependence of the radioprotective effects of human recombinant interleukin-1 alpha, tumor necrosis factor alpha, granulocyte-colony stimulating factor, and murine recombinant granulocyte-colony stimulating factor.J Immunol. 1988;140:108–111.Google Scholar
  20. 20.
    Neta R, Vogel SN, Sipe JD, Wong GG, Nordan RP Comparison of in vivo effects of human recombinant IL-1 and human recombinant IL-6 in mice.Lymphokine Res. 1988;7:403–412.PubMedGoogle Scholar
  21. 21.
    Metcalf D, Nicola NA. Direct proliferative actions of stem cell factor on murine bone marrow cells in vitro: effects of combination with colony stimulating factors.Proc NatlAcad Sci USA. 1991;88:6239–62433.CrossRefGoogle Scholar
  22. 22.
    Neta R, Perlstein R, Vogel SN, Ledney GD, Abrams J. Role of interleukin 6 (IL-6) in protection from lethal irradiation and in endocrine responses to IL-1 and tumor necrosis factor.J Exp Med. 1992;175:689–694.CrossRefPubMedGoogle Scholar
  23. 23.
    Vogel SN, Douches SD, Kaufman EN, Neta R. Induction of colony stimulating factor in vivo by recombinant interleukin-1 alpha and recombinant tumor necrosis factor alpha.J Immunol. 1987;138:2143–21488.PubMedGoogle Scholar
  24. 24.
    Kinnamon KE, Ketterling LL, Stampfli HF, Grenan MM. Mouse endogenous spleen counts as a means of screening for anti-radiation drug.Proc Soc Exp Biol Med. 1980;164:370–373.CrossRefPubMedGoogle Scholar
  25. 25.
    Neta R, Sztein MB, Oppenheim JJ, Gillis S, Douches SD. The in vivo effects of interleukin-1,I: bone marrow cells induced to cycle after administration of interleukin-1.J Immunol. 1987;139:1861–1866.PubMedGoogle Scholar
  26. 26.
    Metcalf D. The molecular biology and functions of the granulocytes macrophage colony stimulating factors.Blood. 1986;67:257–267.PubMedGoogle Scholar
  27. 27.
    Metcalf D, Moore MAS.Haemopoietic Cells. Amsterdam: North Holland; 1971.Frontiers in Biology; vol 24.Google Scholar

Copyright information

© The Japanese Society of Hematology 2003

Authors and Affiliations

  • Jie-Young Song
    • 1
  • Hyun-Ok Yang
    • 2
  • Ji-Young Shim
    • 1
  • Ji-Yeon Ahn
    • 1
  • Young-Soo Han
    • 1
  • In-Sung Jung
    • 1
  • Yeon-Sook Yun
    • 1
  1. 1.Laboratory of ImmunologyKorea Institute of Radiological and Medical SciencesSeoulKorea
  2. 2.Department of Anesthesiology and Pain Medicine, onCollege of MedicineUniversity of UlsanSeoulKorea

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