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International Journal of Hematology

, Volume 78, Issue 3, pp 213–218 | Cite as

High Expression of YB-1 Gene in Erythroid Cells in Patients with Refractory Anemia

  • Hisayuki Yokoyama
  • Hideo Harigae
  • Shinichiro Takahashi
  • Junichi Kameoka
  • Koichi Miyamura
  • Kenichi Ishizawa
  • Mitsuo Kaku
  • Takeshi Sasaki
Progress in Hematology

Abstract

It has been shown that aberrant expression of a transcription factor, GATA-1, leads to maturation arrest and transformation of erythroid cells. We previously reported that a multifunctional protein, YB-1, was expressed strongly in the spleen of a GATA-1 mutant mouse, which was filled with transformed erythroblasts. This finding suggested that YB-1 has roles in ery-thropoiesis. In this study, we examined in vivo expression of YB-1 messenger RNA (mRNA) in bone marrow erythroid cells and erythroid leukemic cell lines. During erythroid differentiation of erythroid leukemic cell lines, the expression level of YB-1 mRNA was highest at the early phase of differentiation and then decreased. In human bone marrow cells, the in vivo expression level of YB-1 mRNA was higher in glycophorin A-positive cells than in glycophorin A-negative cells. An interesting finding was that expression of YB-1 was higher in erythroblasts in myelodysplastic syndrome-refractory anemia (MDS-RA) than in normal cells. The findings suggested that YB-1 functions in the early stage of erythropoiesis and that aberrant expression of this protein may induce hematological diseases such as MDS.

Key words

Erythropoiesis YB-1 gene GATA factors Myelodysplastic syndrome 

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Copyright information

© The Japanese Society of Hematology 2003

Authors and Affiliations

  • Hisayuki Yokoyama
    • 1
  • Hideo Harigae
    • 2
  • Shinichiro Takahashi
    • 1
  • Junichi Kameoka
    • 1
  • Koichi Miyamura
    • 1
  • Kenichi Ishizawa
    • 1
  • Mitsuo Kaku
    • 2
  • Takeshi Sasaki
    • 1
  1. 1.Departments of RheumatologyTohoku University School of MedicineSendaiJapan
  2. 2.Department of Molecular DiagnosticsTohoku University School of MedicineSendaiJapan

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