Abstract
Objective: Preparation of anti-human androgen receptor(hAR) monoclonal antibody (McAb). Methods: Four cells lines of hybridoma secreting specific monoclonal antibodies against AR were first established by fusion SP2/0 cell with spleen cell from BALB/c mice immunized with the coupling complex of hAR-KLH. Results: Paraffin-embedded sections of 45 prostate cancers were detected. There was an overall concordance of 91% using Immunohistochemistry between AR polyclonal antibody from Zymed and hAR-N McAb selfmade. Conclusion: The results show that the McAb obtained in this study would be a useful tool to detect the AR status in prostate cancer.
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Shen, Jh., He, Lw., Liu, Cy. et al. Generation of monoclonal antibody against human androgen receptor with synthetic peptide. Chin J Cancer Res 12, 268–270 (2000). https://doi.org/10.1007/BF02983503
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DOI: https://doi.org/10.1007/BF02983503