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Induction of systemic resistance toColletotrichum falcatum in sugarcane by a synthetic signal molecule, acibenzolar-S-Methyl(CGA-245704)

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Abstract

The effect of a novel synthetic signal molecule, acibenzolar-S-methyl (CGA-245704; benzo [1,2,3] thiadiazole-7-carbothioic acid S-methyl ester), in inducing resistance in sugarcane against red rot disease caused by the fungusColletotrichum falcatum Went was studied. Application of CGA-245704 as a soil drench or along with marcotting rooting mixture induced resistance in sugarcane to challenge inoculation withC. falcatum. When the pathogen was inoculated by the plug method, it caused discoloration in the untreated control stalk tissues; however, in the stalk tissues pretreated with acibenzolar-S-methyl, pathogen colonization was considerably reduced. When the pathogen was inoculated by nodal swabbing, its penetration was arrested in the sensitized stalk tissues. An induced systemic resistance effect was found to persist up to 30 days in the pretreated cut canes. Increased phenolic content and accumulation of pathogenesis-related (PR) proteins,viz., chitinase, β-1,3-glucanase and thaumatin-like protein (PR-5), were observed in sugarcane plants treated with acibenzolar-S-methyl.

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References

  1. Benhamou, N. and Belanger, R.R. (1998) Induction of systemic resistance toPythium damping-off in cucumber plants by benzothiadiazole — infrastructure and cytochemistry of the host response.Plant J. 14:13- 21.

    Article  PubMed  CAS  Google Scholar 

  2. Berger, L.R. and Reynolds, D.M. (1958) The chitinase system of a strain ofStreptomyces griseus. Biochem.Biophys. Acta 29:522–534.

    Article  CAS  Google Scholar 

  3. Bol, J.F., Linthorst, H.J.M. and Cornelessen, B.J.C. (1990) Plant pathogenesis related proteins induced by virus infection.Annu. Rev. Phytopathol. 28:113–138.

    Article  CAS  Google Scholar 

  4. Boiler, T. and Mauch, F. (1988) Colorimetric assay for chitinase.Methods Enzymol, 161:430–435.

    Article  Google Scholar 

  5. Broglie, K., Chet, I., Holliday, M., Cressman, R., Biddle, P., Knowlton, S.et al, (1991) Transgenic plants with enhanced resistance to the fungal pathogenRhizoctonia solani.Science 254:1194–1197.

    Article  PubMed  CAS  Google Scholar 

  6. Carrasco, A., Boudet, A.M. and Marigo, G. (1978) Enhanced resistance of tomato plants toFusarium by controlled stimulation of their natural phenolic production.Physiol. Plant Pathol, 12:225–232.

    Article  CAS  Google Scholar 

  7. Chen, W.P., Chen, P.D., Liu, D.J., Kynast, R., Friebe, B., Velazhahan, R.et al, (1999) Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene.Them. Appl. Genet. 99:755–760.

    Article  CAS  Google Scholar 

  8. Chona, B.L. (1980) Red rot of sugarcane and sugar industry—a review.Indian Phytopathol, 33:191–207.

    Google Scholar 

  9. Cohen, Y., Gisi, U. and Niderman, T. (1993) Local and systemic protection againstPhytophthora infestans induced in potato and tomato plants by jasmonic acid methyl ester.Phytopathology 83:1054–1062.

    Article  CAS  Google Scholar 

  10. Cohen, Y, Reuveni, M. and Baider, A. (1999) Local and systemic activity of BABA (DL-3-aminobutyric acid) againstPlasmopara viticola in grapevines.Eur. J. Plant Pathol. 105:351–361.

    Article  CAS  Google Scholar 

  11. Datta, K., Tu, J., Oliva, N., Ona, I., Velazhahan, R., Mew, T.W.et al. (2001) Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars.Plant Sci. 160:405–414.

    Article  PubMed  CAS  Google Scholar 

  12. Datta, K., Velazhahan, R., Oliva, N., Ona, I., Mew, T, Khush, G.S.et al. (1999) Over expression of cloned rice thaumatin-like protein (PR5) gene in transgenic rice plants enhances environmental friendly resistance toRhizoctonia solani causing sheath blight disease.Theor. Appl, Genet. 98:1138–1145.

    Article  CAS  Google Scholar 

  13. Dean, R.A. and Kuć, J. (1986) Induced systemic protection in cucumber: time of production and movement of the signal.Phytopathology 76:966–970.

    Article  Google Scholar 

  14. Dean, R.A. and Kuć, J. (1986) Induced systemic protection in cucumber: the source of the signal.Physiol, Mol. Plant Pathol. 28:227–233.

    Google Scholar 

  15. Dean, R.A. and Kuć, J. (1987) Rapid lignification in response to wounding and infection as a mechanism for induced systemic protection in cucumber.Physiol. Mol, Plant Pathol, 31:69–81.

    Article  CAS  Google Scholar 

  16. Delany, T.P., Friedrich, L., Kessmann, H., Uknes, S., Vernooij, B., Ward, E.et al, (1994) The molecular biology of systemic acquired resistance:in: Daniels, M. [Ed.] Advances in Molecular Genetics of Plant- Microbe Interactions. Vol. 3, pp. 339–347. Kluwer Academic Press, Dordrecht, the Netherlands.

    Google Scholar 

  17. Dixon, R.A. and Lamb, C.J. (1990) Molecular communication in interactions between plants and microbial pathogens.Annu. Rev. Plant Physiol. Plant Mol. Biol. 41:339–367.

    Article  CAS  Google Scholar 

  18. Enkerli, J., Gisi, U. and Mosinger, E. (1993) Systemic acquired resistance toPhytophthora infestans in tomato and the role of pathogenesis related proteins.Physiol. Mol. Plant Pathol, 43:161–171.

    Article  CAS  Google Scholar 

  19. Enyedi, A.J., Yalpani, N., Silverman, P. and Raskin, I. (1992) Localization conjugation and function of salicylic acid in tobacco during the hypersensitive reaction to tobacco mosaic virus.Proc. Natl. Acad. Sci. USA 89:2480–2484.

    Article  PubMed  CAS  Google Scholar 

  20. Friedrich, L., Lawton, K., Ruess, W., Masner, P., Specker, N., Rella, M.G.et al. (1996) A benzothiadiazole derivative induces systemic acquired resistance in tobacco.Plant J. 10:61–70.

    Article  CAS  Google Scholar 

  21. Gianinnazi, S. (1984) Genetic and molecular aspects of resistance induced by molecules or chemicals.in: Kosuge, T. and Nester, E.W. [Eds.] Plant-Microbe Interactions, Vol. I. Molecular and Genetic Perspectives, pp. 321 -342. Macmillan, New York, NY.

    Google Scholar 

  22. Gorlach, J., Volrath, S., Knauf-Beiter, G., Hengy, G., Beckhove, U., Kogel, K.H.et al. (1996) Benzothia- diazole—a novel class of inducers of systemic acquired resistance activates gene expression and disease resistance in wheat.Plant Cell 8:629–643.

    Article  PubMed  CAS  Google Scholar 

  23. Hammerschmidt, R. (1999) Induced disease resistance: How do induced plants stop pathogens?Physiol. Mol. Plant Pathol. 55:77–84.

    Article  CAS  Google Scholar 

  24. Hoffland, E., Pieterse, C.M.J., Bik, L. and Van Pett, J.A. (1995) Induced systemic resistance in radish is not associated with accumulation of pathogenesis-related proteins.Physiol. Mol. Plant Pathol. 46:309–320.

    Article  CAS  Google Scholar 

  25. Hong, J.K., Hwang, B.K. and Kim, C.H. (1999) Induction of local and systemic resistance toColletotrichum coccodes in pepper plants by DL-β-amino-n-butyric acid.J. Phytopathol. 147:193–198.

    Article  CAS  Google Scholar 

  26. Ishii, H., Tomita, Y, Horio, T., Narusaka, Y, Nakazawa, Y, Nishimura, K.et al. (1999) Induced resistance of acibenzolar-S-methyl (CGA-245704) to cucumber and Japanese pear diseases.Eur. J. Plant Pathol. 105:77- 85.

    Article  CAS  Google Scholar 

  27. Jensen, B.D., Latundedada, A.O., Hudson, D. and Lucas, J.A. (1998) Protection ofBrassica seedlings against downy mildew and damping-off by seed treatment with CGA-245704, an activator of systemic acquired resistance.Pestic. Sci. 52:63–69.

    Article  CAS  Google Scholar 

  28. Kaji, M., Hirata, A., Nikajima, Y, Sugii, S. and Tsujimoto, K. (1997) Control mechanism of new plant activator, CGA-245704 against rice blast.Ann. Phytopathol. Soc. Jpn. 63:222–223.

    Google Scholar 

  29. Kauffmann, S., Legrand, M., Jeoffroy, P. and Fritig, B. (1987) Biological function of pathogenesis-related proteins. Four PR-proteins of tobacco have l,3-β-glucanase activity.EMBO J. 6:3209–3212.

    PubMed  CAS  Google Scholar 

  30. Kessmann, H., Staub, T, Hofmann, C, Maetzke, T., Herzog, J., Ward, E.et al. (1994) Induction of systemic acquired resistance in plants by chemicals.Annu. Rev. Plant Pathol. 32:439–459.

    CAS  Google Scholar 

  31. Kombrink, E. and Somssich, I.E. (1997) Pathogenesis related proteins and plant defence.in: Carroll, G. and Tudzynski, P. [Eds] The Mycota. V, Part A. Plant Relationships. Springer Verlag, Berlin, Germany. pp. 107–128.

    Google Scholar 

  32. Kunz, W., Schurter, R. and Maetzke, T. (1997) The chemistry of BTH plant activators.Pestic. Sci. 50:275- 282.

    Article  CAS  Google Scholar 

  33. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature (Lond.) 227:680–685.

    Article  CAS  Google Scholar 

  34. Lamb, C.J., Lawton, M.A., Dron, M. and Dixon, R.A. (1989) Signals and transduction mechanisms for activation of plant defenses against microbial attack.Cell 56:215–224.

    Article  PubMed  CAS  Google Scholar 

  35. Lawton, K.A., Friedrich, L., Hunt, M., Weymann, K., Delaney, T, Kessmann, H.et al. (1996) Benzothiadi- azole induces resistance inArabidopsis by activation of the systemic acquired resistance signal transduction pathway.Plant J. 10:71–82.

    Article  PubMed  CAS  Google Scholar 

  36. Legrand, M., Kauffmann, S., Jeoffroy, P. and Fritig, B. (1987) Biological function of pathogenesis-related proteins; Four tobacco pathogenesis-related proteins are chitinases.Proc. Natl. Acad. Sci. USA 84:6750–6754.

    Article  PubMed  CAS  Google Scholar 

  37. Lin, W., Anuratha, C.S., Datta, K., Potrykus, I., Muthukrishnan, S. and Datta, S.K.(1995) Genetic engineering of rice for resistance to sheath blight.Bio/Technology 13:686–691.

    Article  CAS  Google Scholar 

  38. Lyon, G.D. and McGill, F.M. (1988) Inhibition of growth ofErwinia carotovora in vitro by phenolics.Potato Res. 31:461–467.

    Article  CAS  Google Scholar 

  39. Mauch, F., Mauch-Mani, B. and Boiler, T. (1998) Antifungal hydrolases in pea tissue. II. Inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase.Plant Physiol. 88:936–942.

    Article  Google Scholar 

  40. Metraux, J.P., Ahl-Goy, P., Staub, T, Speich, T., Steinamann, R., Ryals, J.et al. (1991) Induced resistance in cucumber in response to 2,6-dichloroisonicotinic acid and pathogens.in: Verma, PS. [Ed.] Advances in Molecular Genetics of Plant-Microbe Interactions. Kluwer Academic Publishers, Dordrecht, the Netherlands.Vol. 1, pp. 432–439.

    Google Scholar 

  41. Mohanraj, D., Padmanaban, P., Viswanathan, R. and Alexander, K.C. (1997) Sugarcane screening for red rot resistance.Sugarcane 3:18–23.

    Google Scholar 

  42. Morris, S.W., Vernory, B., Titatarn, S., Starrett, M., Thomas, S., Wiltse, C.C.et al. (1998) Induced resistance responses in maize.Mol. Plant-Microbe Interact. 11:643–658.

    Article  PubMed  CAS  Google Scholar 

  43. Narusaka, Y, Narusaka, M., Horio, T. and Ishii, H. (1999) Comparison of local and systemic induction of acquired disease resistance in cucumber plants treated with benzothiadiazole or salicylic acid.Plant Cell Physiol. 40:388–395.

    PubMed  CAS  Google Scholar 

  44. Oostendorp, M. von, Kessman, H., Friedrich, L., Geissmann, A., Gorlach, J., Hengy, G.et al. (1996) Influence of plant activator Bion and of triazole fungicides on plant defense mechanism.Gesunde Pflanze 48:260–264.

    Google Scholar 

  45. Pan, S.Q., Ye, X.S. and Kuc, J. (1991) A technique for detection of chitrnases, β-1,3-glucanase, and protein patterns after a single separation using polyacylamide gel electrophoresis or isoelectricfocusing.Phytopathology 81:970–974.

    Article  CAS  Google Scholar 

  46. Pieterse, C.M.J., Van Wees, S.C.M., Van Pelt, J.A., Knoester, M, Laan, R., Gerrits, H.et al. (1998) A novel signalling pathway controlling induced systemic resistance inArabidopsis.Plant Cell 10:1571–1580.

    Article  PubMed  CAS  Google Scholar 

  47. Raum, J. (1997) “Bion”—A plant activator also forvegetable gardening.Gemuse (Munchen) 33 (3):185–187.

    Google Scholar 

  48. Reissig, J.L., Strominger, J.L. and Leloir, L.F. (1955) A modified colorimetric method for the estimation of N-acetylamino sugars.J. Biol. Chem. 217:959–966.

    PubMed  CAS  Google Scholar 

  49. Ruess, W., Kunz, W., Staub, T., MGller, K., Poppinger, N., SpeichJ., et al, (1995) Plant activator CGA- 245704, a new technology for disease management.Abstr. XIII Int. Plant Protection Congr. (the Hague, the Netherlands).

  50. Ruess, W., Mueller, K., Knauf-Beiter, G., Kauf, W. and Staub, T. (1996) Plant activator CGA-245704 - an innovative approach for disease control in cereals and tobacco.Proc. Brighton Crop Protection Conf. Pests and Diseases (Brighton, UK), Vol. 1, pp. 53–60.

  51. Ryals, J., Lawton, K.A., Delaney, T.P., Friedrich, L., Kessmann, H., Neuenschwander V.,etal, (1995) Signal transduction in systemic acquired resistance.Proc. Natl. Acad. Sci. USA 92:4202–4205.

    Article  PubMed  CAS  Google Scholar 

  52. Ryals, J.A., Neuenschwander, U.H., Willits, M.G., Molina, A., Steiner, H.Y. and Hunt, M.D. (1996) Systemic acquired resistance.Plant Cell 8:1809–1819.

    Article  PubMed  CAS  Google Scholar 

  53. Schlosser. E. (1997) SAR—a new dimension in plant protection.Arab.J Plant Prot. 15:149–150.

    Google Scholar 

  54. Schlumbaum, A., Mauch, R, Vogeli, V. and Boiler, T. (1986) Plant chitinases are potent inhibitors of fungal growth.Nature (Lond.) 324:365–367.

    Article  CAS  Google Scholar 

  55. Schweizer, P., Schlagenhauf, E., Schaffrath, E., Schaffrath, U. and Dudler, R. (1999) Different patterns of host genes are induced in rice byPseudomonas syringae, a biological inducer of resistance and the chemical inducer benzodiathiazole (BTH).Eur. J. Plant Pathol. 105:659–665.

    Article  CAS  Google Scholar 

  56. Shulaev, V, Leon, J. and Raskin, I. (1996) Is salicylic acid a translocated signal of systemic acquired resistance in tobacco?Plant Cell 7:1691–1701.

    Article  Google Scholar 

  57. Siegrist, J., Winkal, D.G., Kolle, C. and Schmidtke, M. (1997) Chemically induced resistance in green bean against bacterial and fungal pathogens.J. Plant Dis. Prot. 104:599–610.

    CAS  Google Scholar 

  58. Singh, K. and Singh, R.P. (1989) Red rot.in: Ricaud, C.G., Egan, B.T., Gillaspie, A.G. and Hughes, C.G. [Eds.] Sugarcane Diseases of the World: Major Diseases. Elsevier, Amsterdam, the Netherlands. pp. 169–188.

    Google Scholar 

  59. Srinivasan, K.V. and Bhat, N. (1961) Red rot of sugarcane—criteria for grading resistance.J. Indian Bot. Soc. 40:566–577.

    Google Scholar 

  60. Sundara, B. (1998) Sugarcane Cultivation. Vikas Publishing House, New Delhi, India.

    Google Scholar 

  61. Toal, E.S., and Jones, P.W. (1999) Induction of systemic resistance toSclerotinia sclerotiorum by oxalic acid in oilseed rape.Plant Pathol. 48:759–767.

    Article  Google Scholar 

  62. Uknes, S., Mauch-Mani, B., Moyer, M., Potter, S., Williams, S., Dincher, S.et al. (1992) Acquired resistance in arabidopsis.Plant Cell 4:645–656.

    Article  PubMed  CAS  Google Scholar 

  63. Van Loon, L.C., Bakker, P.A.H.M. and Pieterse, C.M.J. (1998) Systemic resistance induced by rhizosphere bacteria.Annu. Rev. Phytopathol. 36:453–483.

    Article  PubMed  Google Scholar 

  64. Velazhahan, R., Datta, S.K. and Muthukrishnan, S. (1999) The PR-5 family; Thaumatin-like proteins,in: Datta, S.K. and Muthukrishnan, S. [Eds.] CRC Press, Boca Raton, FL, USA. pp. 107–129.

    Google Scholar 

  65. Velazhahan, R., Samiyappan, R. and Vidhyasekaran, P. (2000) Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells.Phytoparasitica 28:131–139.

    Article  CAS  Google Scholar 

  66. Vidhyasekaran, P. (1998) Fungal Pathogenesis in Plants and Crops. Marcel Dekker Inc., New York, NY.

    Google Scholar 

  67. Vigers, A.J., Roberts, W.K. and Selitrennikoff, C.P. (1991) A new family of plant antifungal proteins.Mol. Plant-Microbe Interact. 4:315–323.

    PubMed  CAS  Google Scholar 

  68. Viswanathan, R., Mohanraj, D. and Padmanabhan, P. (1998) Comparison of three testing methods for evaluation of resistance to red rot caused byColletotrichum falcatum in sugarcane (Saccharum ojficinarum).Indian J. Agric. Sci. 68:226–230.

    Google Scholar 

  69. Viswanathan, R. and Samiyappan, R. (1999) Red rot disease of sugarcane: Major constraint for Indian sugar industry.Sugarcane 5:9–15.

    Google Scholar 

  70. White, R.F. (1979) Acetylsalicylic acid (aspirin) induces resistance to tobacco mosaic virus in tobacco.Virology 99:410–412.

    Article  PubMed  CAS  Google Scholar 

  71. Zhu, B., Chen, T.H.H. and Li, R.H. (1996) Analysis of late blight disease resistance and freezing tolerance in transgenic potato plants expressing sense and antisense genes for an osmotin-like protein.Planta 198:70–77.

    Article  PubMed  CAS  Google Scholar 

  72. Zieslin, N. and Ben-Zaken, R. (1993) Peroxidase activity and presence of phenolic substances in peduncles of rose flowers.Plant Physiol. Biochem. 31:333–339.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Sugarcane Breeding Institute, ICAR, 641007, Coimbatore

    A. Ramesh Sundar, R. Viswanathan & P. Padmanaban

  2. Tamil Nadu Agricultural University, 641003, Coimbatore, India

    R. Velazhahan & P. Vidhyasekaran

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  1. A. Ramesh Sundar
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Sundar, A.R., Velazhahan, R., Viswanathan, R. et al. Induction of systemic resistance toColletotrichum falcatum in sugarcane by a synthetic signal molecule, acibenzolar-S-Methyl(CGA-245704). Phytoparasitica 29, 231–242 (2001). https://doi.org/10.1007/BF02983455

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