Abstract
Objective: To explore the effects of perioperative cimetidine administration on tumor cell nuclear morphometric parameters and DNA content in patients with gastrointestinal adenocarcinoma. Methods: 49 patients with pathologically confirmed gastrointestinal adenocarcinoma were randomized into test group (n=25) and control group (n=24). The test group started oral cimetidine intake 400 mg, tid, 7–10d before operation, followed by standard curative operation. The control group did not receive cimetidine. Tumor specimens were paraffin embedded for microsection and stained with hematoxylin and eosin (HE) and Feulgen stain. Morphometric studies and DNA content of tumor nuclei were performed on IBAS Image Analyzer. Results: The tumor cell nuclear area (µm2), nuclear perimeter (µm), maximal nuclear diameter (µ) for test group/control group were 23.54 5.08/34.69110.08 (P<0.001), 22.064.43/24.884.05 (P<0.05), 7.8411.64/ 8.6211.24 (P<0.05), 4.4210.61/5.4110.89 (P<0.001), Respectively. The percentages (%) of diploidy, triple-tetraploidy, quintuple ploidy, and >quintuple ploidy tumor cells for test group/control group were 16.6412.58/5.3312.14 (P<0.002), 39.8412.28/35.7013.58 (P>0.50), 12.4215.00/14.4810.74 (P>0.20), 31.1116.86/ 45.9713.82 (P<0.005), respectively. Conclusion: Perioperative administration of cimetidine in gasgtrointestinal cancer patients could decrease the nuclear size and raise the percentage of diploid tumor cells, and convert high aneuploid tumor cells into low-aneuploid tumor cells, which might help reduce the invasiveness of tumor cells.
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References
Adams WJ, Morris DL. Short-course cimetidine and survival with colorectal cancer. Lancet 1994; 344: 1768.
Svendsen LB, Ross C, Knigge U, et al. Cimetidien as an adjuvant treatment in colorectal cancer. Dis Colon Rectum 1995; 38: 514.
Adams WJ, Lawson JA, Morris DL. Cimetidine inhibitsin vivo growth of human colon cancer and reverse histamine stimulatedin vitro andin vivo growth. Gut 1994; 35: 1632.
Li Y, Bai DJ, Wang K, et al. Effects of perioperative cimetidine administration on natural killer cells in patients with gastrointestinal cancer. Chin J Cancer Res 1999; 11: 70.
Qin DX, Ling QH, Wu HW, et al. Image analysis of nuclear DNA content of gastric cancer. Prac J Cancer 1993; 8: 9.
Yi PY, Yang GH, Tang YS, et al. Nuclear morphometric analysis of colorectal adenocarcinoma and its clinicopathologic significance. Chin J Pathol 1994; 23: 141.
Uyterlinde AM, Baak JPA, Schipper NW, et al. Further evaluation of the prognostic value of morphometric and flow cytometric parameters in breast cancer patients with long follow-up. Int J Cancer 1990; 45: 1.
Korenage D, Okamura T, Saito A, et al. DNA ploidy is closely linked to tumor invasion, lymph node metastasis and prognosis in clinical gastric cancer. Cancer 1988; 62: 309.
Watanabe A, Korenage D, Maehara Y, et al. Nuclear DNA content, tumor cell aggregation, and metastatic events in patients with poorly differentiated adenocarcinoma of the stomach. Cancer 1991; 68: 815
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Li, Y., Yang, Gl., Yuan, Hy. et al. Effects of perioperative cimetidine administration on tumor cell nuclear morphometry and DNA content in patients with gastrointestinal cancer. Chin J Cancer Res 12, 148–151 (2000). https://doi.org/10.1007/BF02983445
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DOI: https://doi.org/10.1007/BF02983445