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International Journal of Hematology

, Volume 78, Issue 2, pp 126–132 | Cite as

Ex Vivo Expansion of Human Umbilical Cord Blood CD34+ Cells in a Collagen Bead—Containing 3-Dimensional Culture System

  • Han-Soo Kim
  • Jong Baeck Lim
  • Yoo Hong Min
  • Seung Tae Lee
  • Chuhl Joo Lyu
  • Eun Seok Kim
  • Hyun Ok Kim
Article

Abstract

Self-renewal of stem cells depends on several critical factors, including the hematopoietic microenvironment, interactions with supporting stromal cells, features of the extracellular matrix, hematopoietic growth factors, and cytokines. Our study investigated the role of artificial 3-dimensional microenvironments as a means of replicating a more physiologic milieu in expansion of cord blood CD34+ cells. In the 3-dimensional model, hematopoietic cells inside collagen beads are exposed to cytokines added to a culture medium. We found that amplification of CD34+ cells with a clonogenic assay, fluorescenceactivated cell sorter analysis, and bone marrow repopulation of NOD/SCID mice showed greater clonogenic ability of cells cultured by the 3-dimensional method compared with the 2-dimensional method. The present study demonstrated that 3-dimensional matrix support may be useful for extended periods in expansion and preservation of stem cells or progenitor cells in vitro.

Key words

Cord blood CD34+ hematopoietic stem cell Ex vivo expansion Collagen beads NOD/SCID mouse 

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Copyright information

© The Japanese Society of Hematology 2003

Authors and Affiliations

  • Han-Soo Kim
    • 1
    • 2
  • Jong Baeck Lim
    • 3
  • Yoo Hong Min
    • 2
  • Seung Tae Lee
    • 2
  • Chuhl Joo Lyu
    • 4
  • Eun Seok Kim
    • 5
  • Hyun Ok Kim
    • 3
  1. 1.Brain Korea 21 Project for Medical SciencesUSA
  2. 2.Departments of Internal MedicineUSA
  3. 3.Department of Laboratory MedicineYonsei University College of MedicineSeoulKorea
  4. 4.Pediatrics, Yonsei University, College of MedicineSeoul
  5. 5.Department of Oral and Maxillofacial SurgeryChungnam National University HospitalDaejeonKorea

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