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International Journal of Hematology

, Volume 76, Issue 3, pp 238–243 | Cite as

Expression and Affinity of Homing-Related Molecules on Steady-State Adult and Neonate Human PB CD34+ Cells and Their SRC Activity

  • Fumiya Hirayama
  • Maki Yano
  • Mitsunobu Tanaka
  • Kazuta Yasui
  • Yoshinori Horie
  • Kayoko Matsumoto
  • Nobuo Nagao
  • Yoshihiko Tani
Review Article
  • 30 Downloads

Abstract

Although the vast majority of hematopoietic progenitor cells (HPCs) reside within the bone marrow (BM), a small number of HPCs also continuously circulate in the peripheral blood (PB). The examination of the fate of blood-borne HPCs in parabiotic mice, which are surgically conjoined and share a common circulation, recently revealed that steady-state PB HPCs play a physiological role in, at least, the functional re-engraftment of unconditioned BM. To assess the possibility that human HPCs have a similar function, in this study we examined the expression level and affinity of the homing-related molecules, as well as the SCID mouse reconstituting cell (SRC) activity of human PB CD34+ cells, and compared adults with neonates.There was no remarkable difference between adults and neonates in the expression of E- and/or P-selectin ligands by PB CD34+ cells or in these cells’ affinity to VCAM-1. In contrast, the expression level of CXCR4 on PB CD34+ cells was much lower in adults than in neonates. Adult cells also showed a much lower SRC activity than neonates.These results suggest that human PB HPCs may contribute to steady-state hematopoiesis in the BM of neonates to some extent, but not so much in adults.

Key words

Steady-state PB Homing CXCR4 SRC 

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Copyright information

© The Japanese Society of Hematology 2002

Authors and Affiliations

  • Fumiya Hirayama
    • 1
  • Maki Yano
    • 1
  • Mitsunobu Tanaka
    • 1
  • Kazuta Yasui
    • 1
  • Yoshinori Horie
    • 1
  • Kayoko Matsumoto
    • 1
  • Nobuo Nagao
    • 1
  • Yoshihiko Tani
    • 1
  1. 1.Osaka Red Cross Blood CenterJoto-ku, OsakaJapan

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